S-thanatin enhances the efficacy of tigecycline in an experimental rat model of polymicrobial peritonitis

Oscar Cirioni, Guoqiu Wu, Linxian Li, Fiorenza Orlando, Carmela Silvestri, Roberto Ghiselli, Zilong Shen, Alessandro Scalise, Eleonora Gabrielli, Daniele Scuppa, Chiara Romiti, Mauro Provinciali, Mario Guerrieri, Andrea Giacometti

Research output: Contribution to journalArticlepeer-review

Abstract

We investigated the efficacy of the peptide s-thanatin alone and in combination with tigecycline in an animal model of sepsis induced by cecal ligation and puncture. Adult male Wistar rats were randomized to receive intravenously isotonic sodium chloride solution, 5 mg/kg s-thanatin, 2 mg/kg tigecycline, 5 mg/kg s-thanatin combined with 2 mg/kg tigecycline. The experiment was also performed with administration of the drugs 360 min after the surgical procedure to better investigate the clinical situation where there is an interval between the onset of sepsis and the initiation of therapy. Lethality, bacterial growth in blood, peritoneum, spleen and liver, and NO indices were evaluated. All compounds reduced the lethality when compared to control. In all experiments, the compounds reduced significantly bacterial growth and lethality compared with saline treatment. Treatment with s-thanatin resulted in significant decrease in plasma NO levels compared to tigecycline and control group. The combination between s-thanatin and tigecycline proved to be the most effective treatment in reducing all variables measured. S-thanatin may have potential therapeutic usefulness alone and when associated to tigecycline in polymicrobial peritonitis.

Original languageEnglish
Pages (from-to)1231-1236
Number of pages6
JournalPeptides
Volume31
Issue number7
DOIs
Publication statusPublished - Jul 2010

Keywords

  • Antimicrobial peptides
  • Polymicrobial peritonitis
  • S-thanatin
  • Tigecycline

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

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