S100B maternal blood levels are gestational age- and gender-dependent in healthy pregnancies

Laura D Serpero, Vincenza Bianchi, Francesca Pluchinotta, Erika Conforti, Ekaterina Baryshnikova, Roberto Guaschino, Maurizio Cassinari, Oria Trifoglio, Maria Grazia Calevo, Diego Gazzolo

Research output: Contribution to journalArticlepeer-review


BACKGROUND: S100B is a well-established biomarker of central nervous system (CNS) development and damage in the perinatal period. Because the fetal CNS induces an overproduction of S100B measurable in the maternal bloodstream we evaluated S100B protein in healthy pregnancies in order to provide a reference curve of the protein in the second and third trimesters and to provide information on CNS development when standard monitoring procedures could be silent or unavailable.

METHODS: Between July 2012 and December 2014 we conducted a prospective study in 1213 healthy pregnancies delivering healthy newborns. Maternal blood samples were collected for standard monitoring procedures and S100B assessment. S100B correlations with selected outcomes (gestational age at sampling, gender of fetus, gestational age and weight at birth, delivery mode) were calculated using multiple forward stepwise regression analysis.

RESULTS: S100B concentrations in the second and third trimesters of pregnancy were found to be gestational age-, gender- and delivery mode-dependent (p<0.05, for all). Multiple forward stepwise regression analysis with S100B as the dependent variable and gestational age at sampling, gender, delivery mode, gestational age and weight at birth as independent variables, showed a significant correlation between S100B and gestational age at sampling (R=0.13; p<0.001).

CONCLUSIONS: The present findings offering a S100B protein reference curve in maternal blood suggest that non-invasive fetal CNS monitoring is becoming feasible and open the way to further research in neuro-biomarker assessment in the maternal bloodstream.

Original languageEnglish
Pages (from-to)1770-1776
Number of pages7
JournalClinical Chemistry and Laboratory Medicine
Issue number11
Publication statusPublished - Oct 26 2017


  • Adult
  • Biomarkers
  • Birth Weight
  • Female
  • Gestational Age
  • Humans
  • Immunoassay
  • Luminescent Measurements
  • Male
  • Perinatal Care
  • Pregnancy
  • Prospective Studies
  • Regression Analysis
  • S100 Calcium Binding Protein beta Subunit
  • Sex Factors
  • Young Adult
  • Journal Article


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