S100B protein and 4-hydroxynonenal in the spinal cord of wobbler mice

Valentina Corvino, Rita Businaro, Maria Concetta Geloso, Paolo Bigini, Valentina Cavallo, Elena Pompili, Tiziana Mennini, Lorenzo Fumagalli, Fabrizio Michetti

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

S100B is a calcium-binding protein that, in the nervous system, is mainly concentrated in glial cells. Although its biological role is still unclear, the protein is hypothesized, at high concentrations, to act in the pathogenesis of neurodegenerative processes, possibly through oxidative stress mechanisms. To investigate this hypothesis we studied the spinal cord of wobbler mice, an animal model of motor neuron degeneration. Using immunocytochemistry, we detected an overexpression of S100B in astrocytes of the cervical spinal cord of these animals. We also confirmed this finding by reverse transcriptase polymerase chain reaction. In the same spinal cord regions, scattered neurons appeared to be immunostained for 4-hydroxynonenal-modified proteins, an indicator of lipid peroxidation. This finding constitutes a sign of oxidative stress-induced neurodegeneration.

Original languageEnglish
Pages (from-to)341-345
Number of pages5
JournalNeurochemical Research
Volume28
Issue number2
DOIs
Publication statusPublished - Feb 1 2003

Fingerprint

Spinal Cord
Oxidative Stress
Oxidative stress
Nerve Degeneration
Neurons
Calcium-Binding Proteins
Animals
Motor Neurons
Reverse Transcriptase Polymerase Chain Reaction
Neuroglia
Astrocytes
Nervous System
Lipid Peroxidation
Proteins
Animal Models
Polymerase chain reaction
Immunohistochemistry
RNA-Directed DNA Polymerase
Neurology
Lipids

Keywords

  • 4-hydroxinonenal
  • Neurodegeneration
  • Oxidative stress
  • S100B protein
  • Wobbler mice

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Corvino, V., Businaro, R., Geloso, M. C., Bigini, P., Cavallo, V., Pompili, E., ... Michetti, F. (2003). S100B protein and 4-hydroxynonenal in the spinal cord of wobbler mice. Neurochemical Research, 28(2), 341-345. https://doi.org/10.1023/A:1022345720852

S100B protein and 4-hydroxynonenal in the spinal cord of wobbler mice. / Corvino, Valentina; Businaro, Rita; Geloso, Maria Concetta; Bigini, Paolo; Cavallo, Valentina; Pompili, Elena; Mennini, Tiziana; Fumagalli, Lorenzo; Michetti, Fabrizio.

In: Neurochemical Research, Vol. 28, No. 2, 01.02.2003, p. 341-345.

Research output: Contribution to journalArticle

Corvino, V, Businaro, R, Geloso, MC, Bigini, P, Cavallo, V, Pompili, E, Mennini, T, Fumagalli, L & Michetti, F 2003, 'S100B protein and 4-hydroxynonenal in the spinal cord of wobbler mice', Neurochemical Research, vol. 28, no. 2, pp. 341-345. https://doi.org/10.1023/A:1022345720852
Corvino, Valentina ; Businaro, Rita ; Geloso, Maria Concetta ; Bigini, Paolo ; Cavallo, Valentina ; Pompili, Elena ; Mennini, Tiziana ; Fumagalli, Lorenzo ; Michetti, Fabrizio. / S100B protein and 4-hydroxynonenal in the spinal cord of wobbler mice. In: Neurochemical Research. 2003 ; Vol. 28, No. 2. pp. 341-345.
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