Safety and feasibility of exhaled breath condensate collection in ventilated infants and children

W. G. Muller, F. Morini, S. Eaton, M. Peters, A. Jaffe

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The aim of this study was to develop a technique for the collection of exhaled breath condensate (EBC) from ventilated children and assess its safety and feasibility. Collection of EBC is used to investigate markers of oxidative stress in the lower airway. No studies have assessed its safety in ventilated children. An in vitro model was developed by connecting a ventilator to an artificial lung; 14 clinical and ventilatory parameters were measured during EBC collection from ventilated children. Levels of 8-isoprostane were measured following collection with and without humidification of the inhaled gas. Amount of water vapour collected was linearly related to time and to minute ventilation in the in vitro model. EBC collections (n=68) were made from ventilated children. In the nonhumidified group, the mean (range) positive end-expiratory pressure increased by 4.1% (2.8-5.5%) and the peak inspiratory flow decreased by 6.1% (11.0-1.3%) during collection. Detectable levels of 8-isoprostane were only found in 10 out of 18 nonhumidified EBC samples (median (range) 4.7 pg·mL-1 (0-5.8)). Collection of exhaled breath condensate from ventilated infants and children is feasible and safe. Discontinuation of humidification is likely to be important in standardising the measurement of inflammatory parameters in exhaled breath condensate collected from ventilated children.

Original languageEnglish
Pages (from-to)479-485
Number of pages7
JournalEuropean Respiratory Journal
Volume28
Issue number3
DOIs
Publication statusPublished - Sep 2006

Fingerprint

8-epi-prostaglandin F2alpha
Safety
Positive-Pressure Respiration
Steam
Mechanical Ventilators
Ventilation
Oxidative Stress
Gases
Lung
In Vitro Techniques

Keywords

  • 8-isoprostane
  • Exhaled breath condensate
  • Safety
  • Ventilation

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Safety and feasibility of exhaled breath condensate collection in ventilated infants and children. / Muller, W. G.; Morini, F.; Eaton, S.; Peters, M.; Jaffe, A.

In: European Respiratory Journal, Vol. 28, No. 3, 09.2006, p. 479-485.

Research output: Contribution to journalArticle

Muller, W. G. ; Morini, F. ; Eaton, S. ; Peters, M. ; Jaffe, A. / Safety and feasibility of exhaled breath condensate collection in ventilated infants and children. In: European Respiratory Journal. 2006 ; Vol. 28, No. 3. pp. 479-485.
@article{ac73703474d64a8b8f212f885f399df2,
title = "Safety and feasibility of exhaled breath condensate collection in ventilated infants and children",
abstract = "The aim of this study was to develop a technique for the collection of exhaled breath condensate (EBC) from ventilated children and assess its safety and feasibility. Collection of EBC is used to investigate markers of oxidative stress in the lower airway. No studies have assessed its safety in ventilated children. An in vitro model was developed by connecting a ventilator to an artificial lung; 14 clinical and ventilatory parameters were measured during EBC collection from ventilated children. Levels of 8-isoprostane were measured following collection with and without humidification of the inhaled gas. Amount of water vapour collected was linearly related to time and to minute ventilation in the in vitro model. EBC collections (n=68) were made from ventilated children. In the nonhumidified group, the mean (range) positive end-expiratory pressure increased by 4.1{\%} (2.8-5.5{\%}) and the peak inspiratory flow decreased by 6.1{\%} (11.0-1.3{\%}) during collection. Detectable levels of 8-isoprostane were only found in 10 out of 18 nonhumidified EBC samples (median (range) 4.7 pg·mL-1 (0-5.8)). Collection of exhaled breath condensate from ventilated infants and children is feasible and safe. Discontinuation of humidification is likely to be important in standardising the measurement of inflammatory parameters in exhaled breath condensate collected from ventilated children.",
keywords = "8-isoprostane, Exhaled breath condensate, Safety, Ventilation",
author = "Muller, {W. G.} and F. Morini and S. Eaton and M. Peters and A. Jaffe",
year = "2006",
month = "9",
doi = "10.1183/09031936.06.00063505",
language = "English",
volume = "28",
pages = "479--485",
journal = "Scandinavian Journal of Respiratory Diseases",
issn = "0903-1936",
publisher = "European Respiratory Society",
number = "3",

}

TY - JOUR

T1 - Safety and feasibility of exhaled breath condensate collection in ventilated infants and children

AU - Muller, W. G.

AU - Morini, F.

AU - Eaton, S.

AU - Peters, M.

AU - Jaffe, A.

PY - 2006/9

Y1 - 2006/9

N2 - The aim of this study was to develop a technique for the collection of exhaled breath condensate (EBC) from ventilated children and assess its safety and feasibility. Collection of EBC is used to investigate markers of oxidative stress in the lower airway. No studies have assessed its safety in ventilated children. An in vitro model was developed by connecting a ventilator to an artificial lung; 14 clinical and ventilatory parameters were measured during EBC collection from ventilated children. Levels of 8-isoprostane were measured following collection with and without humidification of the inhaled gas. Amount of water vapour collected was linearly related to time and to minute ventilation in the in vitro model. EBC collections (n=68) were made from ventilated children. In the nonhumidified group, the mean (range) positive end-expiratory pressure increased by 4.1% (2.8-5.5%) and the peak inspiratory flow decreased by 6.1% (11.0-1.3%) during collection. Detectable levels of 8-isoprostane were only found in 10 out of 18 nonhumidified EBC samples (median (range) 4.7 pg·mL-1 (0-5.8)). Collection of exhaled breath condensate from ventilated infants and children is feasible and safe. Discontinuation of humidification is likely to be important in standardising the measurement of inflammatory parameters in exhaled breath condensate collected from ventilated children.

AB - The aim of this study was to develop a technique for the collection of exhaled breath condensate (EBC) from ventilated children and assess its safety and feasibility. Collection of EBC is used to investigate markers of oxidative stress in the lower airway. No studies have assessed its safety in ventilated children. An in vitro model was developed by connecting a ventilator to an artificial lung; 14 clinical and ventilatory parameters were measured during EBC collection from ventilated children. Levels of 8-isoprostane were measured following collection with and without humidification of the inhaled gas. Amount of water vapour collected was linearly related to time and to minute ventilation in the in vitro model. EBC collections (n=68) were made from ventilated children. In the nonhumidified group, the mean (range) positive end-expiratory pressure increased by 4.1% (2.8-5.5%) and the peak inspiratory flow decreased by 6.1% (11.0-1.3%) during collection. Detectable levels of 8-isoprostane were only found in 10 out of 18 nonhumidified EBC samples (median (range) 4.7 pg·mL-1 (0-5.8)). Collection of exhaled breath condensate from ventilated infants and children is feasible and safe. Discontinuation of humidification is likely to be important in standardising the measurement of inflammatory parameters in exhaled breath condensate collected from ventilated children.

KW - 8-isoprostane

KW - Exhaled breath condensate

KW - Safety

KW - Ventilation

UR - http://www.scopus.com/inward/record.url?scp=33751282873&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33751282873&partnerID=8YFLogxK

U2 - 10.1183/09031936.06.00063505

DO - 10.1183/09031936.06.00063505

M3 - Article

VL - 28

SP - 479

EP - 485

JO - Scandinavian Journal of Respiratory Diseases

JF - Scandinavian Journal of Respiratory Diseases

SN - 0903-1936

IS - 3

ER -