Safety of leucodepleted salvaged blood in oncological surgery: an in vitro model

C Marraccini, L Merolle, P Berni, K Boito, I Tamagnini, E Kuhn, M Ragazzi, R Baricchi, T A Pertinhez

Research output: Contribution to journalArticle

Abstract

Intra-operative blood cell salvage (IOCS) is mainly avoided in onco surgery due to the suspicion that it could increase metastasis' risk. We simulated IOCS followed by leucodepletion: HCT116 (human colorectal cancer) cells were inoculated into packed red blood cells units, and their distribution was evaluated, step-by-step, by flow cytometry and immunohistochemistry. Most of HCT116 cells were lost during washing, and almost completely removed after filtration. IOCS plus leucodepletion could be of great advantage for oncological patients, where allogenic blood transfusion could influence tumour progression.

Original languageEnglish
Pages (from-to)803-805
Number of pages3
JournalVox Sanguinis
Volume112
Issue number8
DOIs
Publication statusPublished - Nov 2017

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Operative Blood Salvage
Blood Cells
Safety
HCT116 Cells
Blood Transfusion
Colorectal Neoplasms
Flow Cytometry
Erythrocytes
Immunohistochemistry
Neoplasm Metastasis
In Vitro Techniques
Neoplasms

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Marraccini, C., Merolle, L., Berni, P., Boito, K., Tamagnini, I., Kuhn, E., ... Pertinhez, T. A. (2017). Safety of leucodepleted salvaged blood in oncological surgery: an in vitro model. Vox Sanguinis, 112(8), 803-805. https://doi.org/10.1111/vox.12565

Safety of leucodepleted salvaged blood in oncological surgery : an in vitro model. / Marraccini, C; Merolle, L; Berni, P; Boito, K; Tamagnini, I; Kuhn, E; Ragazzi, M; Baricchi, R; Pertinhez, T A.

In: Vox Sanguinis, Vol. 112, No. 8, 11.2017, p. 803-805.

Research output: Contribution to journalArticle

Marraccini, C, Merolle, L, Berni, P, Boito, K, Tamagnini, I, Kuhn, E, Ragazzi, M, Baricchi, R & Pertinhez, TA 2017, 'Safety of leucodepleted salvaged blood in oncological surgery: an in vitro model', Vox Sanguinis, vol. 112, no. 8, pp. 803-805. https://doi.org/10.1111/vox.12565
Marraccini C, Merolle L, Berni P, Boito K, Tamagnini I, Kuhn E et al. Safety of leucodepleted salvaged blood in oncological surgery: an in vitro model. Vox Sanguinis. 2017 Nov;112(8):803-805. https://doi.org/10.1111/vox.12565
Marraccini, C ; Merolle, L ; Berni, P ; Boito, K ; Tamagnini, I ; Kuhn, E ; Ragazzi, M ; Baricchi, R ; Pertinhez, T A. / Safety of leucodepleted salvaged blood in oncological surgery : an in vitro model. In: Vox Sanguinis. 2017 ; Vol. 112, No. 8. pp. 803-805.
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