Salivary alpha-synuclein in the diagnosis of Parkinson's disease and Progressive Supranuclear Palsy

Giorgio Vivacqua, Antonio Suppa, Romina Mancinelli, Daniele Belvisi, Andrea Fabbrini, Matteo Costanzo, Alessandra Formica, Paolo Onori, Giovanni Fabbrini, Alfredo Berardelli

Research output: Contribution to journalArticle

Abstract

INTRODUCTION: Alpha-synuclein (α-syn) aggregation is the pathological hallmark of Parkinson's Disease (PD). In this study, we measured α-syn total (α-syntotal), oligomeric α-syn (α-synolig) and α-synolig/α-syntotal ratio in the saliva of patients affected by PD and in age and sex-matched healthy subjects. We also compared salivary α-syntotal measured in PD with those detected in Progressive Supranuclear Palsy (PSP), in order to assess whether salivary α-syn can be used as a biomarker for PD and for the differential diagnosis between PD and PSP.

METHODS: We studied 100 PD patients, 20 patients affected by PSP and 80 age- and sex-matched healthy subjects. ELISA analysis was performed using two commercial ELISA platforms and a specific ELISA assay for α-syn aggregates.

RESULTS: We detected lower α-syntotal and higher α-synolig in PD than in healthy subjects. Conversely in PSP salivary α-syntotal concentration was comparable to that measured in healthy subjects. Receiver Operating Characteristic analyses revealed specific cut-off values able to differentiate PD patients from healthy subjects and PSP patients with high sensitivity and specificity. However, there was no significant correlation between clinical and molecular data.

CONCLUSION: Salivary α-syn detection could be a promising and easily accessible biomarker for PD and for the differential diagnosis between PD and PSP.

Original languageEnglish
Pages (from-to)143-148
Number of pages6
JournalParkinsonism and Related Disorders
Volume63
DOIs
Publication statusPublished - Jun 2019

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