Salmeterol inhibits the allergen-induced mononuclear cell proliferation and downregulates GM-CSF release and HLA-DR expression by monocytes

S. Oddera, M. Silvestri, L. Scarso, R. Testi, G. A. Rossi

Research output: Contribution to journalArticle

Abstract

β2-adrenoceptor agonists are known to attenuate several functions of human mononuclear cells in response to activation. Since monocytes and lymphocytes play a major pathogenetic role in allergic asthma, this study was designed to evaluate the hypothesis that salmeterol, a β2-adrenoceptor agonist with a long duration of action, could inhibit in vitro the allergen-induced activation of blood mononuclear cells (BMCs). BMCs were collected from subjects sensitized to Dermatophagoides pteronyssinus (Der p) and incubated with a purified Der p allergen extract to evaluate the ability of salmeterol to downregulate: (a) BMC proliferation; (b) IL-2 receptors (r) and HLA-DR surface antigen tag) expression; (c) cytokine release. Der p induced a significant BMC proliferation (P <0.01), associated with increased expression of HLA-DRag and IL-2r (P <0.05) and with enhanced release of IL-2, GM-CSF, IL-1β, TNF-α and IFN-γ (P <0.01, each comparison). Salmeterol (10-8-10-6 M) significantly inhibited, in a dose dependent manner, the Der p-induced BMC proliferation, reducing (at 10-7 M) HLA-DRag expression on monocytes and GM-CSF release (P <0.05, each comparison). These data demonstrate that β2-adrenoceptor-mediated suppression of allergen-induced BMC activation is associated with inhibition of cytokine release and of surface molecule expression, which are involved in the interaction between T-lymphocytes and antigen-presenting cells.

Original languageEnglish
Pages (from-to)43-49
Number of pages7
JournalPulmonary Pharmacology and Therapeutics
Volume10
Issue number1
DOIs
Publication statusPublished - Feb 1997

Keywords

  • Cytokines
  • Interleukin-2 receptor (IL-2r)
  • MHC class II molecules
  • Mononuclear cells
  • Salmeterol

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Pharmacology

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