TY - JOUR
T1 - Sample preparation and orthogonal chromatography for broad polarity range plasma metabolomics
T2 - Application to human subjects with neurodegenerative dementia
AU - Armirotti, Andrea
AU - Basit, Abdul
AU - Realini, Natalia
AU - Caltagirone, Carlo
AU - Bossù, Paola
AU - Spalletta, Gianfranco
AU - Piomelli, Daniele
PY - 2014/6/15
Y1 - 2014/6/15
N2 - We describe a simple protocol for the preparation and orthogonal hydrophobic/hydrophilic LC-MS/MS analysis of mouse and human plasma samples, which enables the untargeted ("shotgun") or targeted profiling of hydrophilic, amphipathic, and hydrophobic constituents of plasma metabolome. The protocol is rapid, efficient, and reliable, and offers several advantages compared to current procedures. When applied to a training set of human plasma samples, the protocol allowed for the rapid acquisition of full LogP metabolic profiles in plasma samples obtained from cognitively healthy human subjects and age-matched subjects with mild cognitive impairment or Alzheimer's disease (n = 15 each). Targeted analyses confirmed these findings, which are consistent with data previously published by other groups.
AB - We describe a simple protocol for the preparation and orthogonal hydrophobic/hydrophilic LC-MS/MS analysis of mouse and human plasma samples, which enables the untargeted ("shotgun") or targeted profiling of hydrophilic, amphipathic, and hydrophobic constituents of plasma metabolome. The protocol is rapid, efficient, and reliable, and offers several advantages compared to current procedures. When applied to a training set of human plasma samples, the protocol allowed for the rapid acquisition of full LogP metabolic profiles in plasma samples obtained from cognitively healthy human subjects and age-matched subjects with mild cognitive impairment or Alzheimer's disease (n = 15 each). Targeted analyses confirmed these findings, which are consistent with data previously published by other groups.
KW - Dried plasma spot
KW - Mass spectrometry
KW - Metabolomics
KW - Orthogonal chromatography
KW - Sample preparation
UR - http://www.scopus.com/inward/record.url?scp=84899410319&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84899410319&partnerID=8YFLogxK
U2 - 10.1016/j.ab.2014.03.019
DO - 10.1016/j.ab.2014.03.019
M3 - Article
C2 - 24708938
AN - SCOPUS:84899410319
VL - 455
SP - 48
EP - 54
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 1
ER -