Sample preparation and orthogonal chromatography for broad polarity range plasma metabolomics: Application to human subjects with neurodegenerative dementia

Andrea Armirotti, Abdul Basit, Natalia Realini, Carlo Caltagirone, Paola Bossù, Gianfranco Spalletta, Daniele Piomelli

Research output: Contribution to journalArticlepeer-review

Abstract

We describe a simple protocol for the preparation and orthogonal hydrophobic/hydrophilic LC-MS/MS analysis of mouse and human plasma samples, which enables the untargeted ("shotgun") or targeted profiling of hydrophilic, amphipathic, and hydrophobic constituents of plasma metabolome. The protocol is rapid, efficient, and reliable, and offers several advantages compared to current procedures. When applied to a training set of human plasma samples, the protocol allowed for the rapid acquisition of full LogP metabolic profiles in plasma samples obtained from cognitively healthy human subjects and age-matched subjects with mild cognitive impairment or Alzheimer's disease (n = 15 each). Targeted analyses confirmed these findings, which are consistent with data previously published by other groups.

Original languageEnglish
Pages (from-to)48-54
Number of pages7
JournalAnalytical Biochemistry
Volume455
Issue number1
DOIs
Publication statusPublished - Jun 15 2014

Keywords

  • Dried plasma spot
  • Mass spectrometry
  • Metabolomics
  • Orthogonal chromatography
  • Sample preparation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology
  • Medicine(all)

Fingerprint Dive into the research topics of 'Sample preparation and orthogonal chromatography for broad polarity range plasma metabolomics: Application to human subjects with neurodegenerative dementia'. Together they form a unique fingerprint.

Cite this