Sample preparation and orthogonal chromatography for broad polarity range plasma metabolomics: Application to human subjects with neurodegenerative dementia

Andrea Armirotti; Abdul Basit; Natalia Realini; Carlo Caltagirone; Paola Bossù; Gianfranco Spalletta; Daniele Piomelli

doi:10.1016/j.ab.2014.03.019

Sample preparation and orthogonal chromatography for broad polarity range plasma metabolomics: Application to human subjects with neurodegenerative dementia

Andrea Armirotti, Abdul Basit, Natalia Realini, Carlo Caltagirone, Paola Bossù, Gianfranco Spalletta, Daniele Piomelli

Fondazione Santa Lucia

Research output: Contribution to journal › Article › peer-review

Abstract

We describe a simple protocol for the preparation and orthogonal hydrophobic/hydrophilic LC-MS/MS analysis of mouse and human plasma samples, which enables the untargeted ("shotgun") or targeted profiling of hydrophilic, amphipathic, and hydrophobic constituents of plasma metabolome. The protocol is rapid, efficient, and reliable, and offers several advantages compared to current procedures. When applied to a training set of human plasma samples, the protocol allowed for the rapid acquisition of full LogP metabolic profiles in plasma samples obtained from cognitively healthy human subjects and age-matched subjects with mild cognitive impairment or Alzheimer's disease (n = 15 each). Targeted analyses confirmed these findings, which are consistent with data previously published by other groups.

Original language	English
Pages (from-to)	48-54
Number of pages	7
Journal	Analytical Biochemistry
Volume	455
Issue number	1
DOIs	https://doi.org/10.1016/j.ab.2014.03.019
Publication status	Published - Jun 15 2014

Keywords

Dried plasma spot
Mass spectrometry
Metabolomics
Orthogonal chromatography
Sample preparation

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology
Cell Biology
Medicine(all)

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Sample preparation and orthogonal chromatography for broad polarity range plasma metabolomics : Application to human subjects with neurodegenerative dementia. / Armirotti, Andrea; Basit, Abdul; Realini, Natalia; Caltagirone, Carlo ; Bossù, Paola ; Spalletta, Gianfranco; Piomelli, Daniele.

In: Analytical Biochemistry, Vol. 455, No. 1, 15.06.2014, p. 48-54.

Research output: Contribution to journal › Article › peer-review

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