Sanger sequencing in routine KRAS testing: A review of 1720 cases from a pathologist's perspective

Umberto Malapelle, Claudio Bellevicine, Maria Salatiello, Caterina De Luca, Elisabetta Rispo, Palmira Riccio, Lucianna Sparano, Alfonso De Stefano, Chiara Carlomagno, Francesco Maria Maiello, Giulia Vita, Oscar Nappi, Giancarlo Troncone

Research output: Contribution to journalArticle

Abstract

Background: Sanger sequencing (SS) of PCR products is still the most frequent method to test colorectal cancer for KRAS mutations in routine practice. Methods: An audit of SS on 1720 routine cases was carried out, taking into account age, gender, specimen type (resection vs biopsies), tumour site ( primary vs metastasis), tumour stage, neoplastic cells abundance (>30% vs

Original languageEnglish
Pages (from-to)940-944
Number of pages5
JournalJournal of Clinical Pathology
Volume65
Issue number10
DOIs
Publication statusPublished - Oct 2012

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Colorectal Neoplasms
Neoplasms
Neoplasm Metastasis
Biopsy
Polymerase Chain Reaction
Mutation
Pathologists

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Malapelle, U., Bellevicine, C., Salatiello, M., De Luca, C., Rispo, E., Riccio, P., ... Troncone, G. (2012). Sanger sequencing in routine KRAS testing: A review of 1720 cases from a pathologist's perspective. Journal of Clinical Pathology, 65(10), 940-944. https://doi.org/10.1136/jclinpath-2012-200773

Sanger sequencing in routine KRAS testing : A review of 1720 cases from a pathologist's perspective. / Malapelle, Umberto; Bellevicine, Claudio; Salatiello, Maria; De Luca, Caterina; Rispo, Elisabetta; Riccio, Palmira; Sparano, Lucianna; De Stefano, Alfonso; Carlomagno, Chiara; Maiello, Francesco Maria; Vita, Giulia; Nappi, Oscar; Troncone, Giancarlo.

In: Journal of Clinical Pathology, Vol. 65, No. 10, 10.2012, p. 940-944.

Research output: Contribution to journalArticle

Malapelle, U, Bellevicine, C, Salatiello, M, De Luca, C, Rispo, E, Riccio, P, Sparano, L, De Stefano, A, Carlomagno, C, Maiello, FM, Vita, G, Nappi, O & Troncone, G 2012, 'Sanger sequencing in routine KRAS testing: A review of 1720 cases from a pathologist's perspective', Journal of Clinical Pathology, vol. 65, no. 10, pp. 940-944. https://doi.org/10.1136/jclinpath-2012-200773
Malapelle, Umberto ; Bellevicine, Claudio ; Salatiello, Maria ; De Luca, Caterina ; Rispo, Elisabetta ; Riccio, Palmira ; Sparano, Lucianna ; De Stefano, Alfonso ; Carlomagno, Chiara ; Maiello, Francesco Maria ; Vita, Giulia ; Nappi, Oscar ; Troncone, Giancarlo. / Sanger sequencing in routine KRAS testing : A review of 1720 cases from a pathologist's perspective. In: Journal of Clinical Pathology. 2012 ; Vol. 65, No. 10. pp. 940-944.
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