Sarcoglycans and gabaa receptors in rat central nervous system: An immunohistochemical study

Giuseppina Cutroneo, Placido Bramanti, Giuseppe Anastasi, Daniele Bruschetta, Angelo Favaloro, Giovanna Vermiglio, Fabio Trimarchi, Debora Di Mauro, Giuseppina Rizzo

Research output: Contribution to journalArticle

Abstract

Sarcoglycan subcomplex is a transmembrane glycoprotein system which connects extracellular matrix to cytoskeleton. Although this complex has been found in several non-muscular tissues, no data exist about a sarcoglycan subcomplex in brain. Only the presence of å-sarcoglycan in brain has been described in detail because its mutation determines Myoclonus Dystonia Syndrome. Also æ-, β- and δ-sarcoglycans have been found in brain but only at mRNA level and their distribution in brain is still unknown. Here, we have searched for the expression of all sarcoglycans in specific brain regions of rat as hippocampus, cerebral and cerebellar cortex. Since a correlation between dystrophin glycoprotein complex and ã-amino butyric acid A (GABAA) receptor was demonstrated, we have investigated also a possible colocalization between sarcoglycans and GABAA receptor. Results have shown that all sarcoglycans are expressed in neurons of all observed regions; these proteins show a spot-like pattern of fluorescence and are mainly localized at soma level. Moreover, each sarcoglycan colocalizes with GABAA receptor. The present study shows, for the first time, the expression of all sarcoglycans in brain; moreover, the prevalent localization of sarcoglycans at post-synaptic level and the colocalization of these glycoproteins with GABAA receptor suggests that sarcoglycans play a key role in central nervous system, regulating post-synaptic receptors assembly.

Original languageEnglish
Pages (from-to)105-116
Number of pages12
JournalItalian journal of anatomy and embryology = Archivio italiano di anatomia ed embriologia
Volume120
Issue number2
DOIs
Publication statusPublished - 2015

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Keywords

  • Cerebellar cortex
  • GABA receptor
  • Neurons
  • Rat
  • Sarcoglycans

ASJC Scopus subject areas

  • Anatomy
  • Embryology

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