SARG: A new human osteosarcoma cell line. Expression of bone markers and of major histocompatibility antigens

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A new cell line (SARG) was established from a human radiation-induced osteosarcoma (OSA). It showed an epithelial-like morphology with polimorphous and sometimes bizarre nuclei. SARG had an osteoblastic differentiation pattern: almost 100% of the cells were positive for alkaline phosphatase, type I and III collagens and osteonectin. The expression of class I HLA antigens was detectable even after 40 in vitro passages. The expression of MHC antigens was greatly increased after in vitro treatment with interferon gamma (IFN-γ), whereas interferon alpha (IFN-α) and tumor necrosis factor alpha (TNF-α) increased the expression of class I antigens, but not of class II antigens. SARG was tumorigenic after subcutaneous injection in nude mice. Experimental metastases were never detected.

Original languageEnglish
Pages (from-to)29-31
Number of pages3
JournalAnnals of Oncology
Issue numberSUPPL. 2
Publication statusPublished - 1992


ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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