Abstract
A new cell line (SARG) was established from a human radiation-induced osteosarcoma (OSA). It showed an epithelial-like morphology with polimorphous and sometimes bizarre nuclei. SARG had an osteoblastic differentiation pattern: almost 100% of the cells were positive for alkaline phosphatase, type I and III collagens and osteonectin. The expression of class I HLA antigens was detectable even after 40 in vitro passages. The expression of MHC antigens was greatly increased after in vitro treatment with interferon gamma (IFN-γ), whereas interferon alpha (IFN-α) and tumor necrosis factor alpha (TNF-α) increased the expression of class I antigens, but not of class II antigens. SARG was tumorigenic after subcutaneous injection in nude mice. Experimental metastases were never detected.
Original language | English |
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Pages (from-to) | 29-31 |
Number of pages | 3 |
Journal | Annals of Oncology |
Volume | 3 |
Issue number | SUPPL. 2 |
Publication status | Published - 1992 |
ASJC Scopus subject areas
- Cancer Research
- Oncology