Satellite cell characterization from aging human muscle

Arianna Corbu, Annarita Scaramozza, Lucilla Badiali-DeGiorgi, Lucia Tarantino, Valentina Papa, Rita Rinaldi, Roberto D'Alessandro, Marcello Zavatta, Massimo Laus, Giovanna Lattanzi, Giovanna Cenacchi

Research output: Contribution to journalArticle

Abstract

Objectives: Satellite cells (SCs) are skeletal muscle progenitor cells located between the basal lamina and the sarcolemma of muscle fibers. They are responsible for muscle growth and repair. In humans, aging results in the depletion of the SC population and in its proliferative activity, but not in its function. It has not yet been determined whether under conditions of massive muscle fiber death in vivo, the regenerative potential of SCs is totally or partially compromised in old muscle. No studies have yet tested whether advanced age is a factor that restrains the response of SCs to muscle denervation in humans; this is also due to difficulties in the isolation and in the culture of SCs from a small human surgery fragment. The aim of this study was to study in depth muscle regeneration analysing the SC ability of SCs to proliferate and differentiate in aging human patients. Methods: In order to study in more detail the molecular mechanism, the proliferative and differentiative ability of aging SCs, we isolated SCs from aging human muscle biopsies and analysed their morphology by transmission electron microscopy and immunocytochemical analysis (antibodies against desmin, N-CAM and M-cadherin) and their capacity to grow and to expand in vitro. Moreover, in order to evaluate gene expression of myogenic regulatory factors Myf5, MyoD and myogenin (Myf4), RT-PCR was performed. Results and discussion: SCs isolated from aging human muscle biopsies and plated into favorable proliferation and differentiation conditions were able to proceed through the myogenic program and actively form myotubes, although taking longer than the young control sample. The RT-PCR analysis together with the ultrastructural SC features showed that the myogenic potential seemed to be compromised during the aging human muscle proliferation in vitro.

Original languageEnglish
Pages (from-to)63-72
Number of pages10
JournalNeurological Research
Volume32
Issue number1
DOIs
Publication statusPublished - Feb 2010

Fingerprint

Cell Aging
Muscles
Myogenic Regulatory Factors
Muscle Denervation
Myogenin
Biopsy
Polymerase Chain Reaction
Sarcolemma
Desmin
Skeletal Muscle Fibers
Transmission Electron Microscopy
Basement Membrane
Muscle Cells
Regeneration
Stem Cells
Cell Culture Techniques
Gene Expression
Antibodies
Growth
Population

Keywords

  • Aging muscle
  • Culture of SCs
  • Human surgery fragment
  • Isolation of satellite cell
  • Satellite cell

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology

Cite this

Corbu, A., Scaramozza, A., Badiali-DeGiorgi, L., Tarantino, L., Papa, V., Rinaldi, R., ... Cenacchi, G. (2010). Satellite cell characterization from aging human muscle. Neurological Research, 32(1), 63-72. https://doi.org/10.1179/174313209X385725

Satellite cell characterization from aging human muscle. / Corbu, Arianna; Scaramozza, Annarita; Badiali-DeGiorgi, Lucilla; Tarantino, Lucia; Papa, Valentina; Rinaldi, Rita; D'Alessandro, Roberto; Zavatta, Marcello; Laus, Massimo; Lattanzi, Giovanna; Cenacchi, Giovanna.

In: Neurological Research, Vol. 32, No. 1, 02.2010, p. 63-72.

Research output: Contribution to journalArticle

Corbu, A, Scaramozza, A, Badiali-DeGiorgi, L, Tarantino, L, Papa, V, Rinaldi, R, D'Alessandro, R, Zavatta, M, Laus, M, Lattanzi, G & Cenacchi, G 2010, 'Satellite cell characterization from aging human muscle', Neurological Research, vol. 32, no. 1, pp. 63-72. https://doi.org/10.1179/174313209X385725
Corbu A, Scaramozza A, Badiali-DeGiorgi L, Tarantino L, Papa V, Rinaldi R et al. Satellite cell characterization from aging human muscle. Neurological Research. 2010 Feb;32(1):63-72. https://doi.org/10.1179/174313209X385725
Corbu, Arianna ; Scaramozza, Annarita ; Badiali-DeGiorgi, Lucilla ; Tarantino, Lucia ; Papa, Valentina ; Rinaldi, Rita ; D'Alessandro, Roberto ; Zavatta, Marcello ; Laus, Massimo ; Lattanzi, Giovanna ; Cenacchi, Giovanna. / Satellite cell characterization from aging human muscle. In: Neurological Research. 2010 ; Vol. 32, No. 1. pp. 63-72.
@article{7760e09715544b9bbfd1f7c54ed810f6,
title = "Satellite cell characterization from aging human muscle",
abstract = "Objectives: Satellite cells (SCs) are skeletal muscle progenitor cells located between the basal lamina and the sarcolemma of muscle fibers. They are responsible for muscle growth and repair. In humans, aging results in the depletion of the SC population and in its proliferative activity, but not in its function. It has not yet been determined whether under conditions of massive muscle fiber death in vivo, the regenerative potential of SCs is totally or partially compromised in old muscle. No studies have yet tested whether advanced age is a factor that restrains the response of SCs to muscle denervation in humans; this is also due to difficulties in the isolation and in the culture of SCs from a small human surgery fragment. The aim of this study was to study in depth muscle regeneration analysing the SC ability of SCs to proliferate and differentiate in aging human patients. Methods: In order to study in more detail the molecular mechanism, the proliferative and differentiative ability of aging SCs, we isolated SCs from aging human muscle biopsies and analysed their morphology by transmission electron microscopy and immunocytochemical analysis (antibodies against desmin, N-CAM and M-cadherin) and their capacity to grow and to expand in vitro. Moreover, in order to evaluate gene expression of myogenic regulatory factors Myf5, MyoD and myogenin (Myf4), RT-PCR was performed. Results and discussion: SCs isolated from aging human muscle biopsies and plated into favorable proliferation and differentiation conditions were able to proceed through the myogenic program and actively form myotubes, although taking longer than the young control sample. The RT-PCR analysis together with the ultrastructural SC features showed that the myogenic potential seemed to be compromised during the aging human muscle proliferation in vitro.",
keywords = "Aging muscle, Culture of SCs, Human surgery fragment, Isolation of satellite cell, Satellite cell",
author = "Arianna Corbu and Annarita Scaramozza and Lucilla Badiali-DeGiorgi and Lucia Tarantino and Valentina Papa and Rita Rinaldi and Roberto D'Alessandro and Marcello Zavatta and Massimo Laus and Giovanna Lattanzi and Giovanna Cenacchi",
year = "2010",
month = "2",
doi = "10.1179/174313209X385725",
language = "English",
volume = "32",
pages = "63--72",
journal = "Neurological Research",
issn = "0161-6412",
publisher = "Maney Publishing",
number = "1",

}

TY - JOUR

T1 - Satellite cell characterization from aging human muscle

AU - Corbu, Arianna

AU - Scaramozza, Annarita

AU - Badiali-DeGiorgi, Lucilla

AU - Tarantino, Lucia

AU - Papa, Valentina

AU - Rinaldi, Rita

AU - D'Alessandro, Roberto

AU - Zavatta, Marcello

AU - Laus, Massimo

AU - Lattanzi, Giovanna

AU - Cenacchi, Giovanna

PY - 2010/2

Y1 - 2010/2

N2 - Objectives: Satellite cells (SCs) are skeletal muscle progenitor cells located between the basal lamina and the sarcolemma of muscle fibers. They are responsible for muscle growth and repair. In humans, aging results in the depletion of the SC population and in its proliferative activity, but not in its function. It has not yet been determined whether under conditions of massive muscle fiber death in vivo, the regenerative potential of SCs is totally or partially compromised in old muscle. No studies have yet tested whether advanced age is a factor that restrains the response of SCs to muscle denervation in humans; this is also due to difficulties in the isolation and in the culture of SCs from a small human surgery fragment. The aim of this study was to study in depth muscle regeneration analysing the SC ability of SCs to proliferate and differentiate in aging human patients. Methods: In order to study in more detail the molecular mechanism, the proliferative and differentiative ability of aging SCs, we isolated SCs from aging human muscle biopsies and analysed their morphology by transmission electron microscopy and immunocytochemical analysis (antibodies against desmin, N-CAM and M-cadherin) and their capacity to grow and to expand in vitro. Moreover, in order to evaluate gene expression of myogenic regulatory factors Myf5, MyoD and myogenin (Myf4), RT-PCR was performed. Results and discussion: SCs isolated from aging human muscle biopsies and plated into favorable proliferation and differentiation conditions were able to proceed through the myogenic program and actively form myotubes, although taking longer than the young control sample. The RT-PCR analysis together with the ultrastructural SC features showed that the myogenic potential seemed to be compromised during the aging human muscle proliferation in vitro.

AB - Objectives: Satellite cells (SCs) are skeletal muscle progenitor cells located between the basal lamina and the sarcolemma of muscle fibers. They are responsible for muscle growth and repair. In humans, aging results in the depletion of the SC population and in its proliferative activity, but not in its function. It has not yet been determined whether under conditions of massive muscle fiber death in vivo, the regenerative potential of SCs is totally or partially compromised in old muscle. No studies have yet tested whether advanced age is a factor that restrains the response of SCs to muscle denervation in humans; this is also due to difficulties in the isolation and in the culture of SCs from a small human surgery fragment. The aim of this study was to study in depth muscle regeneration analysing the SC ability of SCs to proliferate and differentiate in aging human patients. Methods: In order to study in more detail the molecular mechanism, the proliferative and differentiative ability of aging SCs, we isolated SCs from aging human muscle biopsies and analysed their morphology by transmission electron microscopy and immunocytochemical analysis (antibodies against desmin, N-CAM and M-cadherin) and their capacity to grow and to expand in vitro. Moreover, in order to evaluate gene expression of myogenic regulatory factors Myf5, MyoD and myogenin (Myf4), RT-PCR was performed. Results and discussion: SCs isolated from aging human muscle biopsies and plated into favorable proliferation and differentiation conditions were able to proceed through the myogenic program and actively form myotubes, although taking longer than the young control sample. The RT-PCR analysis together with the ultrastructural SC features showed that the myogenic potential seemed to be compromised during the aging human muscle proliferation in vitro.

KW - Aging muscle

KW - Culture of SCs

KW - Human surgery fragment

KW - Isolation of satellite cell

KW - Satellite cell

UR - http://www.scopus.com/inward/record.url?scp=77949470763&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77949470763&partnerID=8YFLogxK

U2 - 10.1179/174313209X385725

DO - 10.1179/174313209X385725

M3 - Article

C2 - 20092696

AN - SCOPUS:77949470763

VL - 32

SP - 63

EP - 72

JO - Neurological Research

JF - Neurological Research

SN - 0161-6412

IS - 1

ER -