Saturating the region of the polycystic kidney disease gene with NotI linking clones

Heinz Himmelbauer, Greg G. Germino, Isabella Ceccherini, Giovanni Romeo, Stephen T. Reeders, Anna Maria Frischauf

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A NotI-linking library was constructed from a radiation hybrid containing fragments of human chromosome 16. The clones were mapped on a panel of somatic cell hybrids, and 10 different NotI site-containing clones were localized close to and between genetic markers flanking the PKD1 locus. With pulsed-field gel analysis the clones were shown to be distributed over four adjacent ClaI fragments covering 1,200 kb.

Original languageEnglish
Pages (from-to)325-334
Number of pages10
JournalAmerican Journal of Human Genetics
Volume48
Issue number2
Publication statusPublished - Feb 1991

Fingerprint

Polycystic Kidney Diseases
Clone Cells
Radiation Hybrid Mapping
Genes
Chromosomes, Human, Pair 16
Hybrid Cells
Human Chromosomes
Genetic Markers
Libraries
Gels

ASJC Scopus subject areas

  • Genetics

Cite this

Himmelbauer, H., Germino, G. G., Ceccherini, I., Romeo, G., Reeders, S. T., & Frischauf, A. M. (1991). Saturating the region of the polycystic kidney disease gene with NotI linking clones. American Journal of Human Genetics, 48(2), 325-334.

Saturating the region of the polycystic kidney disease gene with NotI linking clones. / Himmelbauer, Heinz; Germino, Greg G.; Ceccherini, Isabella; Romeo, Giovanni; Reeders, Stephen T.; Frischauf, Anna Maria.

In: American Journal of Human Genetics, Vol. 48, No. 2, 02.1991, p. 325-334.

Research output: Contribution to journalArticle

Himmelbauer, H, Germino, GG, Ceccherini, I, Romeo, G, Reeders, ST & Frischauf, AM 1991, 'Saturating the region of the polycystic kidney disease gene with NotI linking clones', American Journal of Human Genetics, vol. 48, no. 2, pp. 325-334.
Himmelbauer H, Germino GG, Ceccherini I, Romeo G, Reeders ST, Frischauf AM. Saturating the region of the polycystic kidney disease gene with NotI linking clones. American Journal of Human Genetics. 1991 Feb;48(2):325-334.
Himmelbauer, Heinz ; Germino, Greg G. ; Ceccherini, Isabella ; Romeo, Giovanni ; Reeders, Stephen T. ; Frischauf, Anna Maria. / Saturating the region of the polycystic kidney disease gene with NotI linking clones. In: American Journal of Human Genetics. 1991 ; Vol. 48, No. 2. pp. 325-334.
@article{3c283d013e2f48d6b4f68e4045ffa298,
title = "Saturating the region of the polycystic kidney disease gene with NotI linking clones",
abstract = "A NotI-linking library was constructed from a radiation hybrid containing fragments of human chromosome 16. The clones were mapped on a panel of somatic cell hybrids, and 10 different NotI site-containing clones were localized close to and between genetic markers flanking the PKD1 locus. With pulsed-field gel analysis the clones were shown to be distributed over four adjacent ClaI fragments covering 1,200 kb.",
author = "Heinz Himmelbauer and Germino, {Greg G.} and Isabella Ceccherini and Giovanni Romeo and Reeders, {Stephen T.} and Frischauf, {Anna Maria}",
year = "1991",
month = "2",
language = "English",
volume = "48",
pages = "325--334",
journal = "American Journal of Human Genetics",
issn = "0002-9297",
publisher = "Cell Press",
number = "2",

}

TY - JOUR

T1 - Saturating the region of the polycystic kidney disease gene with NotI linking clones

AU - Himmelbauer, Heinz

AU - Germino, Greg G.

AU - Ceccherini, Isabella

AU - Romeo, Giovanni

AU - Reeders, Stephen T.

AU - Frischauf, Anna Maria

PY - 1991/2

Y1 - 1991/2

N2 - A NotI-linking library was constructed from a radiation hybrid containing fragments of human chromosome 16. The clones were mapped on a panel of somatic cell hybrids, and 10 different NotI site-containing clones were localized close to and between genetic markers flanking the PKD1 locus. With pulsed-field gel analysis the clones were shown to be distributed over four adjacent ClaI fragments covering 1,200 kb.

AB - A NotI-linking library was constructed from a radiation hybrid containing fragments of human chromosome 16. The clones were mapped on a panel of somatic cell hybrids, and 10 different NotI site-containing clones were localized close to and between genetic markers flanking the PKD1 locus. With pulsed-field gel analysis the clones were shown to be distributed over four adjacent ClaI fragments covering 1,200 kb.

UR - http://www.scopus.com/inward/record.url?scp=0026023529&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026023529&partnerID=8YFLogxK

M3 - Article

C2 - 1990840

AN - SCOPUS:0026023529

VL - 48

SP - 325

EP - 334

JO - American Journal of Human Genetics

JF - American Journal of Human Genetics

SN - 0002-9297

IS - 2

ER -