Satureja montana L. Essential Oils: Chemical Profiles/Phytochemical Screening, Antimicrobial Activity and O/W NanoEmulsion Formulations

Alessandro Maccelli, Luca Vitanza, Anna Imbriano, Caterina Fraschetti, Antonello Filippi, Paola Goldoni, Linda Maurizi, Maria Grazia Ammendolia, Maria Elisa Crestoni, Simonetta Fornarini, Luigi Menghini, Maria Carafa, Carlotta Marianecci, Catia Longhi, Federica Rinaldi

Research output: Contribution to journalArticlepeer-review

Abstract

Chemical fingerprints of four different Satureja montana L. essential oils (SEOs) were assayed by an untargeted metabolomics approach based on Fourier-transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) coupled with either electrospray ionization or atmospheric pressure chemical ionization ion sources. Analysis and relative quantification of the non-polar volatile fraction were conducted by gas chromatography (GC) coupled to MS. FT-ICR MS confirmed significant differences in the polar metabolite composition, while GC-MS analyses confirmed slight fluctuations in the relative amount of major terpenes and terpenoids, known to play a key role in antimicrobial mechanisms. Oil in eater (O/W) nanoemulsions (NEs) composed by SEOs and Tween 20 or Tween 80 were prepared and analyzed in terms of hydrodynamic diameter, ζ-potential and polydispersity index. The results confirm the formation of stable NEs homogeneous in size. Minimum inhibitory and minimum bactericidal concentrations of SEOs were determined towards Gram-positive (Listeria monocytogenes, Staphylococcus aureus, Staphylococcus haemolyticus) and Gram-negative clinical isolates (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Serratia marcescens). Commercial SEO showed strongest antibacterial activity, while SEO 3 was found to be the most active among the lab made extractions. MIC and MBC values ranged from 0.39 to 6.25 mg·mL-1. Furthermore, a SEO structured in NEs formulation was able to preserve and improve antimicrobial activity.

Original languageEnglish
JournalPharmaceutics
Volume12
Issue number1
DOIs
Publication statusPublished - Dec 19 2019

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