Abstract
We describe and apply two raster scanning algorithms that are suited for fast imaging and for spectroscopy in a two photon microscope. Imaging can be performed at a rate of 1-100 Hz per line with a closed loop piezo-actuator. In order to reach the single molecule sensitivity and to study the dynamics of the fluorescence emission, the detection is performed via avalanche photodiodes. In a slow scanning algorithm we have implemented photon counting histogram and lifetime analysis on the image. In this way we are able to discriminate between local concentration and molecular brightness and to measure lifetimes on extended samples.
Original language | English |
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Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
Editors | A. Periasmy, P.T.C. So |
Pages | 319-326 |
Number of pages | 8 |
Volume | 5323 |
DOIs | |
Publication status | Published - 2004 |
Event | Progress in Biomedical Optics and Imaging - Multiphoton Microscopy in the Biomedical Sciences IV - San Jose, CA, United States Duration: Jan 25 2004 → Jan 27 2004 |
Other
Other | Progress in Biomedical Optics and Imaging - Multiphoton Microscopy in the Biomedical Sciences IV |
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Country/Territory | United States |
City | San Jose, CA |
Period | 1/25/04 → 1/27/04 |
Keywords
- Fluorescence microscopy
- Intensity distributions
- Photon counting histogram
- Single molecules
- Two-photon excitation
ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Condensed Matter Physics