Scanning SAXS-WAXS microscopy on osteoarthritis-affected bone - An age-related study

C. Giannini, D. Siliqi, M. Ladisa, D. Altamura, A. Diaz, A. Beraudi, T. Sibillano, L. De Caro, S. Stea, F. Baruffaldi, O. Bunk

Research output: Contribution to journalArticle

Abstract

Osteoarthritis (OA), among other bone pathologies, is expected to determine supramolecular changes at the level of the mineralized collagen fiber. In a proof-of-principle study, bone biopsies were collected from six coxarthritis-affected patients, aged 62-87 years, during hip prosthesis implant surgery, sliced down to 100 μm-thick tissues, and investigated using scanning small-angle and wide-angle X-ray scattering (SAXS and WAXS) transmission microscopy. A multi-modal imaging evaluation of the SAXS and WAXS data, combined with principal component and canonical correlation analyses, allowed the transformation of the raw data into microscopy images and inspection of the nanoscale structure of the mineralized collagen fibers across mm2 tissue areas. The combined scanning SAXS and WAXS microscopy is shown to be a suitable choice for characterizing and quantifying the nanostructural properties of collagen over extended areas. The results suggest the existence of a correlation between age and cross-linking-induced rigidity of collagen fibers.

Original languageEnglish
Pages (from-to)110-117
Number of pages8
JournalJournal of Applied Crystallography
Volume47
Issue number1
DOIs
Publication statusPublished - Feb 2014

Fingerprint

Osteoarthritis
Microscopy
Microscopic examination
Bone
Collagen
Scanning
Bone and Bones
Fibers
Tissue
Hip prostheses
Hip Prosthesis
Biopsy
Pathology
X ray scattering
Rigidity
Surgery
Prostheses and Implants
Inspection
X-Rays
Imaging techniques

Keywords

  • bone pathology
  • collagen
  • imaging
  • microscopy
  • osteoarthritis
  • small-angle and wide-angle X-ray scattering

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Giannini, C., Siliqi, D., Ladisa, M., Altamura, D., Diaz, A., Beraudi, A., ... Bunk, O. (2014). Scanning SAXS-WAXS microscopy on osteoarthritis-affected bone - An age-related study. Journal of Applied Crystallography, 47(1), 110-117. https://doi.org/10.1107/S1600576713030215

Scanning SAXS-WAXS microscopy on osteoarthritis-affected bone - An age-related study. / Giannini, C.; Siliqi, D.; Ladisa, M.; Altamura, D.; Diaz, A.; Beraudi, A.; Sibillano, T.; De Caro, L.; Stea, S.; Baruffaldi, F.; Bunk, O.

In: Journal of Applied Crystallography, Vol. 47, No. 1, 02.2014, p. 110-117.

Research output: Contribution to journalArticle

Giannini, C, Siliqi, D, Ladisa, M, Altamura, D, Diaz, A, Beraudi, A, Sibillano, T, De Caro, L, Stea, S, Baruffaldi, F & Bunk, O 2014, 'Scanning SAXS-WAXS microscopy on osteoarthritis-affected bone - An age-related study', Journal of Applied Crystallography, vol. 47, no. 1, pp. 110-117. https://doi.org/10.1107/S1600576713030215
Giannini, C. ; Siliqi, D. ; Ladisa, M. ; Altamura, D. ; Diaz, A. ; Beraudi, A. ; Sibillano, T. ; De Caro, L. ; Stea, S. ; Baruffaldi, F. ; Bunk, O. / Scanning SAXS-WAXS microscopy on osteoarthritis-affected bone - An age-related study. In: Journal of Applied Crystallography. 2014 ; Vol. 47, No. 1. pp. 110-117.
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