Insights into the molecular and cellular mechanisms underlying CNS development have been obtained by placing immature CNS cells ectopically into the brain. Our previous short-term survival experiments demonstrated that when immature cells were transplanted into the developing Nervous System the majority of the cells aggregated into clusters but a small proportion dispersed into the host brain. In this report we followed the behaviour of those donor cells found scattered into the host parenchyma at heterotopic sites. To this end, either freshly dissociated primary cells or conditionally immortalized cells were labelled with BrdU and DiI, and then 3 x 104 cells were injected into the CNS of E15-16 embryos via in utero surgery. Transplanted animals were perfused three weeks after the surgery. Immunohistochemical procedures and analyses of the morphology of the DiI labelled cells indicate that transplanted cells found scattered into the host brain integrate into the embryonic environment and differentiate into neurons and glial cells.
|Number of pages||9|
|Journal||Neuroscience Research Communications|
|Publication status||Published - 1996|
- CNS development
- CNS precursors
- Intracerebral grafting
ASJC Scopus subject areas