Scavenging effects of terbinafine on free radicals in vitro

E. Camera, C. Cannistraci, S. Briganti, D. Colombo, Mauro Picardo

Research output: Contribution to journalArticle

Abstract

One of the goals of antifungal therapy is to combine an anti- inflammatory activity with antimycotic properties, and therefore it is interesting to evaluate the capacity of active antifungal drugs to interfere with different phases of the inflammatory reaction. In order to identify a possible scavenger property of free radical species of the antifungal agent terbinafine, we studied its activity on the reduction of nitrotetrazolium blue chloride (NTB), induced by superoxide anions with the ultraviolet (UV)- and chemical-induced peroxidation of unsaturated lipid targets. NTB reduction was followed by spectrophotometer and the decomposition of squalene or methyl arachidonate by gas chromatography-mass spectrometry. Terbinafine (20 μg and over) was capable of significantly inhibiting NTB reduction, indicating that the drug scavenges superoxide anion radicals. In these conditions no modifications of the concentration of the drug, as evaluated by high performance liquid chromatography, were observed. The UV- or chemically induced peroxidation of squalene and arachidonic acid was significantly reduced in the presence of 50 μg of terbinafine, suggesting that the substance interferes with the chemical properties of peroxyl radicals. In all the tests used the degree of inhibition was proportional to the amount of free radicals generated. In conclusion our results indicate that terbinafine, at therapeutic concentrations, can be considered to be a free radical interceptor in vitro and could exert a mild anti-inflammatory activity in vivo.

Original languageEnglish
Pages (from-to)640-644
Number of pages5
JournalBritish Journal of Dermatology
Volume140
Issue number4
DOIs
Publication statusPublished - 1999

Keywords

  • Anti-inflammatory
  • Free radicals
  • Terbinafine

ASJC Scopus subject areas

  • Dermatology

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