TY - JOUR
T1 - Schlafen 11 assessment in high-grade serous ovarian carcinoma phenotypic and histological distribution
AU - Ferraioli, Domenico
AU - Peñuela, Leonardo
AU - Garuti, Anna
AU - Carrozzi, Chiara
AU - Olivier, Timothee
AU - Spina, Bruno
AU - Salvi, Sandra
AU - Ferrero, Simone
AU - Tille, Jean Christophe
AU - Labidi-Galy, Sana Intidhar
AU - Cirmena, Gabriella
AU - Ballestero, Alberto
AU - Chopin, Nicolas
AU - Beurrier, Frederic
AU - Treilleux, Isabelle
AU - Ray-Coquard, Isabelle
AU - Zoppoli, Gabriele
AU - Vellone, Valerio Gaetano
PY - 2018/10/1
Y1 - 2018/10/1
N2 - OBJECTIVE: To develop a protocol for immunohistochemistry (IHC) to determine the Schlafen 11 (SLFN11) expression in formalinfixed, paraffinembedded (FFPE) highgrade serous ovarian carcinoma (HGSOC) samples. STUDY DESIGN: We validated a reliable SLFN11 antibody (SLFN11Ab) in IHC choosing between 2 antiSLFN11Abs used for western blot through the development of a SLFN11IHC protocol in culture cell block (CCB) of ovarian carcinoma and in an HGSOC tissue microarray (TMA) series. We applied our protocol to a case series of HGSOC samples to confirm our preliminary results. For each case we evaluated both the intensity score (IS) and the distribution score (DS). A final histological score (HS) was obtained as follows: HS=IS×DS. RESULTS: In CCB and TMA series, the antibody #1 at 1:100 dilution was more reliable. This last one was used in our case series to confirm our IHC protocol. DS showed the following results: 27 cases were no stain, 11 cases showed staining for SLFN11 in <10% of cells, 16 cases showed staining in 10–40% of cells, and the remaining 15 cases showed stain in >40% of cells. IS showed the following results: 25 cases were no stain, in 19 cases a dominant weak stain, in 14 cases a moderate stain, and in 11 cases a strong stain. HS for SLFN11 expression presents a normal distribution with a prevalent (≈60%) intermediate expression. CONCLUSION: We develop a reproducible and standardized IHC protocol to determinate the SLFN11 expression in FFPE HGSOC samples using a modified western blot antiSLFN11Ab.
AB - OBJECTIVE: To develop a protocol for immunohistochemistry (IHC) to determine the Schlafen 11 (SLFN11) expression in formalinfixed, paraffinembedded (FFPE) highgrade serous ovarian carcinoma (HGSOC) samples. STUDY DESIGN: We validated a reliable SLFN11 antibody (SLFN11Ab) in IHC choosing between 2 antiSLFN11Abs used for western blot through the development of a SLFN11IHC protocol in culture cell block (CCB) of ovarian carcinoma and in an HGSOC tissue microarray (TMA) series. We applied our protocol to a case series of HGSOC samples to confirm our preliminary results. For each case we evaluated both the intensity score (IS) and the distribution score (DS). A final histological score (HS) was obtained as follows: HS=IS×DS. RESULTS: In CCB and TMA series, the antibody #1 at 1:100 dilution was more reliable. This last one was used in our case series to confirm our IHC protocol. DS showed the following results: 27 cases were no stain, 11 cases showed staining for SLFN11 in <10% of cells, 16 cases showed staining in 10–40% of cells, and the remaining 15 cases showed stain in >40% of cells. IS showed the following results: 25 cases were no stain, in 19 cases a dominant weak stain, in 14 cases a moderate stain, and in 11 cases a strong stain. HS for SLFN11 expression presents a normal distribution with a prevalent (≈60%) intermediate expression. CONCLUSION: We develop a reproducible and standardized IHC protocol to determinate the SLFN11 expression in FFPE HGSOC samples using a modified western blot antiSLFN11Ab.
KW - Anti-SLFN11 antibody
KW - Histological score
KW - Immunohistochemistry
KW - Ovarian cancer
KW - Ovarian neoplasms
KW - Schlafen-11
KW - Serous high grade ovarian cancer.
KW - SLFN11
UR - http://www.scopus.com/inward/record.url?scp=85057314491&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85057314491&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85057314491
VL - 40
SP - 222
EP - 230
JO - Bulletin of the John Rylands Library
JF - Bulletin of the John Rylands Library
SN - 2054-9318
IS - 5
ER -