Schwann cell-specific JAM-C-deficient mice reveal novel expression and functions for JAM-C in peripheral nerves

Bartomeu Colom, Yannick Poitelon, Wenlong Huang, Abigail Woodfin, Sharon Averill, Ubaldo Del Carro, Desirée Zambroni, Susan D. Brain, Mauro Perretti, Amrita Ahluwalia, John V. Priestley, Triantafyllos Chavakis, Beat A. Imhof, M. Laura Feltri, Sussan Nourshargh

Research output: Contribution to journalArticle

Abstract

Junctional adhesion molecule-C (JAM-C) is an adhesion molecule expressed at junctions between adjacent endothelial and epithelial cells and implicated in multiple inflammatory and vascular responses. In addition, we recently reported on the expression of JAM-C in Schwann cells (SCs) and its importance for the integrity and function of peripheral nerves. To investigate the role of JAM-C in neuronal functions further, mice with a specific deletion of JAM-C in SCs (JAM-C SC KO) were generated. Compared to wild-type (WT) controls, JAM-C SC KO mice showed electrophysiological defects, muscular weakness, and hypersensitivity to mechanical stimuli. In addressing the underlying cause of these defects, nerves from JAM-C SC KO mice were found to have morphological defects in the paranodal region, exhibiting increased nodal length as compared to WTs. The study also reports on previously undetected expressions of JAM-C, namely on perineural cells, and in line with nociception defects of the JAM-C SC KO animals, on finely myelinated sensory nerve fibers. Collectively, the generation and characterization of JAM-C SC KO mice has provided unequivocal evidence for the involvement of SC JAM-C in the fine organization of peripheral nerves and in modulating multiple neuronal responses.

Original languageEnglish
Pages (from-to)1064-1076
Number of pages13
JournalFASEB Journal
Volume26
Issue number3
DOIs
Publication statusPublished - Mar 2012

Fingerprint

Junctional Adhesion Molecule C
Schwann Cells
Peripheral Nerves
Cells
Defects
Cell adhesion
Cell Adhesion Molecules
Myelinated Nerve Fibers
Nociception
Muscle Weakness
Endothelial cells

Keywords

  • Adhesion molecules
  • Peripheral nerves
  • Tight junctions

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology

Cite this

Schwann cell-specific JAM-C-deficient mice reveal novel expression and functions for JAM-C in peripheral nerves. / Colom, Bartomeu; Poitelon, Yannick; Huang, Wenlong; Woodfin, Abigail; Averill, Sharon; Del Carro, Ubaldo; Zambroni, Desirée; Brain, Susan D.; Perretti, Mauro; Ahluwalia, Amrita; Priestley, John V.; Chavakis, Triantafyllos; Imhof, Beat A.; Feltri, M. Laura; Nourshargh, Sussan.

In: FASEB Journal, Vol. 26, No. 3, 03.2012, p. 1064-1076.

Research output: Contribution to journalArticle

Colom, B, Poitelon, Y, Huang, W, Woodfin, A, Averill, S, Del Carro, U, Zambroni, D, Brain, SD, Perretti, M, Ahluwalia, A, Priestley, JV, Chavakis, T, Imhof, BA, Feltri, ML & Nourshargh, S 2012, 'Schwann cell-specific JAM-C-deficient mice reveal novel expression and functions for JAM-C in peripheral nerves', FASEB Journal, vol. 26, no. 3, pp. 1064-1076. https://doi.org/10.1096/fj.11-196220
Colom, Bartomeu ; Poitelon, Yannick ; Huang, Wenlong ; Woodfin, Abigail ; Averill, Sharon ; Del Carro, Ubaldo ; Zambroni, Desirée ; Brain, Susan D. ; Perretti, Mauro ; Ahluwalia, Amrita ; Priestley, John V. ; Chavakis, Triantafyllos ; Imhof, Beat A. ; Feltri, M. Laura ; Nourshargh, Sussan. / Schwann cell-specific JAM-C-deficient mice reveal novel expression and functions for JAM-C in peripheral nerves. In: FASEB Journal. 2012 ; Vol. 26, No. 3. pp. 1064-1076.
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