Screening and mutational improvement of a D-ribose secreting Candida pelliculosa strain

Peter De Wulf; Wim Soetaert; Dieter Schwengers; Erick Jerome Vandamme

doi:10.1016/0922-338X(96)89446-3

Screening and mutational improvement of a D-ribose secreting Candida pelliculosa strain

Peter De Wulf, Wim Soetaert, Dieter Schwengers, Erick Jerome Vandamme

Istituto Europeo di Oncologia

Research output: Contribution to journal › Article › peer-review

Abstract

The application of D-ribose in chemistry and pharmacy lately has received much attention. In order to develop an efficient D-ribose production process, a screening strategy was established to isolate naturally occurring D-ribose secreting microorganisms. Of the more than two thousand strains isolated, only four excreted D-ribose in a range from 0.04 to 0.36 g/l, starting from 30 g/l of D-glucose. One strain, identified as Candida pelliculosa, quantitatively co-produced D-arabitol (up to 7 g/l). This strain was subsequently submitted to UV-mutagenesis and a selective nystatin-based enrichment, leading to the isolation of a D-arabitol non-secreting mutant (C. pelliculosa PDW 43 ARA^-), which synthesized 5 g/l of D-ribose from 30 g/l of D-glucose.

Original language	English
Pages (from-to)	1-7
Number of pages	7
Journal	Journal of Fermentation and Bioengineering
Volume	82
Issue number	1
DOIs	https://doi.org/10.1016/0922-338X(96)89446-3
Publication status	Published - 1996

Keywords

Candida pelliculosa
D-arabitol
D-ribose
mutation
screening
transketolase

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

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10.1016/0922-338X(96)89446-3

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title = "Screening and mutational improvement of a D-ribose secreting Candida pelliculosa strain",

abstract = "The application of D-ribose in chemistry and pharmacy lately has received much attention. In order to develop an efficient D-ribose production process, a screening strategy was established to isolate naturally occurring D-ribose secreting microorganisms. Of the more than two thousand strains isolated, only four excreted D-ribose in a range from 0.04 to 0.36 g/l, starting from 30 g/l of D-glucose. One strain, identified as Candida pelliculosa, quantitatively co-produced D-arabitol (up to 7 g/l). This strain was subsequently submitted to UV-mutagenesis and a selective nystatin-based enrichment, leading to the isolation of a D-arabitol non-secreting mutant (C. pelliculosa PDW 43 ARA-), which synthesized 5 g/l of D-ribose from 30 g/l of D-glucose.",

keywords = "Candida pelliculosa, D-arabitol, D-ribose, mutation, screening, transketolase",

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AU - Schwengers, Dieter

AU - Vandamme, Erick Jerome

PY - 1996

Y1 - 1996

N2 - The application of D-ribose in chemistry and pharmacy lately has received much attention. In order to develop an efficient D-ribose production process, a screening strategy was established to isolate naturally occurring D-ribose secreting microorganisms. Of the more than two thousand strains isolated, only four excreted D-ribose in a range from 0.04 to 0.36 g/l, starting from 30 g/l of D-glucose. One strain, identified as Candida pelliculosa, quantitatively co-produced D-arabitol (up to 7 g/l). This strain was subsequently submitted to UV-mutagenesis and a selective nystatin-based enrichment, leading to the isolation of a D-arabitol non-secreting mutant (C. pelliculosa PDW 43 ARA-), which synthesized 5 g/l of D-ribose from 30 g/l of D-glucose.

AB - The application of D-ribose in chemistry and pharmacy lately has received much attention. In order to develop an efficient D-ribose production process, a screening strategy was established to isolate naturally occurring D-ribose secreting microorganisms. Of the more than two thousand strains isolated, only four excreted D-ribose in a range from 0.04 to 0.36 g/l, starting from 30 g/l of D-glucose. One strain, identified as Candida pelliculosa, quantitatively co-produced D-arabitol (up to 7 g/l). This strain was subsequently submitted to UV-mutagenesis and a selective nystatin-based enrichment, leading to the isolation of a D-arabitol non-secreting mutant (C. pelliculosa PDW 43 ARA-), which synthesized 5 g/l of D-ribose from 30 g/l of D-glucose.

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Screening and mutational improvement of a D-ribose secreting Candida pelliculosa strain

Abstract

Keywords

ASJC Scopus subject areas

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