Abstract
The application of D-ribose in chemistry and pharmacy lately has received much attention. In order to develop an efficient D-ribose production process, a screening strategy was established to isolate naturally occurring D-ribose secreting microorganisms. Of the more than two thousand strains isolated, only four excreted D-ribose in a range from 0.04 to 0.36 g/l, starting from 30 g/l of D-glucose. One strain, identified as Candida pelliculosa, quantitatively co-produced D-arabitol (up to 7 g/l). This strain was subsequently submitted to UV-mutagenesis and a selective nystatin-based enrichment, leading to the isolation of a D-arabitol non-secreting mutant (C. pelliculosa PDW 43 ARA-), which synthesized 5 g/l of D-ribose from 30 g/l of D-glucose.
Original language | English |
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Pages (from-to) | 1-7 |
Number of pages | 7 |
Journal | Journal of Fermentation and Bioengineering |
Volume | 82 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1996 |
Keywords
- Candida pelliculosa
- D-arabitol
- D-ribose
- mutation
- screening
- transketolase
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology