Abstract
Completely diverting portacaval shunt (Eck's fistula) in dogs causes hepatocyte atrophy, disruption of hepatocyte organelles, fatty infiltration and lowgrade hyperplasia. The effect of hepatic growth regulatory substances on these changes was assessed by constantly infusing test substances for four postoperative days after Eck's fistula into the detached left portal vein above the shunt. The directly infused left lobes were compared histopathologically with the untreated right lobes. In what has been called an hepatotrophic effect, stimulatory substances prevented the atrophy and increased hepatocyte mitoses. Of the hormones tested, only insulin was strongly hepatotrophic; T3 had a minor effect, and glucagon, prolactin, angiotensin II, vasopressin, norepinephrine and estradiol were inert. Insulin-like growth factor, hepatic stimulatory substance, transforming growth factor-α and hepatocyte growth factor (also known as hematopoietin A) were powerfully hepatotrophic, but epidermal growth factor had a barely discernible effect. Transforming growth factor-β was inhibitory, but tamoxifen, interleukin-1 and interleukin-2 had no effect. The hepatotrophic action of insulin was not altered when the insulin infusate was mixed with transforming growth factor-β or tamoxifen. These experimens show the importance of in vivo in addition to in vitro testing of putative growth control factors. They illustrate how Eck's fistula model can be used to screen for such substances and possibly to help delineate their mechanisms of action.
| Original language | English |
|---|---|
| Pages (from-to) | 665-670 |
| Number of pages | 6 |
| Journal | Hepatology |
| Volume | 14 |
| Issue number | 4 PART 1 |
| Publication status | Published - Oct 1991 |
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ASJC Scopus subject areas
- Hepatology
Cite this
Screening for candidate hepatic growth factors by selective portal infusion after canine Eck's fistula. / Francavilla, Antonio; Starzl, Thomas E.; Porter, Ken; Foglieni, Carlo Scotti; Michalopoulos, George K.; Carrieri, Guiseppe; Trejo, Jose; Azzarone, Alessandro; Barone, Michele; Zeng, Qi Hua.
In: Hepatology, Vol. 14, No. 4 PART 1, 10.1991, p. 665-670.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Screening for candidate hepatic growth factors by selective portal infusion after canine Eck's fistula
AU - Francavilla, Antonio
AU - Starzl, Thomas E.
AU - Porter, Ken
AU - Foglieni, Carlo Scotti
AU - Michalopoulos, George K.
AU - Carrieri, Guiseppe
AU - Trejo, Jose
AU - Azzarone, Alessandro
AU - Barone, Michele
AU - Zeng, Qi Hua
PY - 1991/10
Y1 - 1991/10
N2 - Completely diverting portacaval shunt (Eck's fistula) in dogs causes hepatocyte atrophy, disruption of hepatocyte organelles, fatty infiltration and lowgrade hyperplasia. The effect of hepatic growth regulatory substances on these changes was assessed by constantly infusing test substances for four postoperative days after Eck's fistula into the detached left portal vein above the shunt. The directly infused left lobes were compared histopathologically with the untreated right lobes. In what has been called an hepatotrophic effect, stimulatory substances prevented the atrophy and increased hepatocyte mitoses. Of the hormones tested, only insulin was strongly hepatotrophic; T3 had a minor effect, and glucagon, prolactin, angiotensin II, vasopressin, norepinephrine and estradiol were inert. Insulin-like growth factor, hepatic stimulatory substance, transforming growth factor-α and hepatocyte growth factor (also known as hematopoietin A) were powerfully hepatotrophic, but epidermal growth factor had a barely discernible effect. Transforming growth factor-β was inhibitory, but tamoxifen, interleukin-1 and interleukin-2 had no effect. The hepatotrophic action of insulin was not altered when the insulin infusate was mixed with transforming growth factor-β or tamoxifen. These experimens show the importance of in vivo in addition to in vitro testing of putative growth control factors. They illustrate how Eck's fistula model can be used to screen for such substances and possibly to help delineate their mechanisms of action.
AB - Completely diverting portacaval shunt (Eck's fistula) in dogs causes hepatocyte atrophy, disruption of hepatocyte organelles, fatty infiltration and lowgrade hyperplasia. The effect of hepatic growth regulatory substances on these changes was assessed by constantly infusing test substances for four postoperative days after Eck's fistula into the detached left portal vein above the shunt. The directly infused left lobes were compared histopathologically with the untreated right lobes. In what has been called an hepatotrophic effect, stimulatory substances prevented the atrophy and increased hepatocyte mitoses. Of the hormones tested, only insulin was strongly hepatotrophic; T3 had a minor effect, and glucagon, prolactin, angiotensin II, vasopressin, norepinephrine and estradiol were inert. Insulin-like growth factor, hepatic stimulatory substance, transforming growth factor-α and hepatocyte growth factor (also known as hematopoietin A) were powerfully hepatotrophic, but epidermal growth factor had a barely discernible effect. Transforming growth factor-β was inhibitory, but tamoxifen, interleukin-1 and interleukin-2 had no effect. The hepatotrophic action of insulin was not altered when the insulin infusate was mixed with transforming growth factor-β or tamoxifen. These experimens show the importance of in vivo in addition to in vitro testing of putative growth control factors. They illustrate how Eck's fistula model can be used to screen for such substances and possibly to help delineate their mechanisms of action.
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UR - http://www.scopus.com/inward/citedby.url?scp=0026077226&partnerID=8YFLogxK
M3 - Article
C2 - 1916668
AN - SCOPUS:0026077226
VL - 14
SP - 665
EP - 670
JO - Hepatology
JF - Hepatology
SN - 0270-9139
IS - 4 PART 1
ER -