Screening of a microvascular endothelial cDNA library identifies rabaptin 5 as a novel autoantigen in Alzheimer's disease

Federica Delunardo; Paola Margutti; Simona Pontecorvo; Tania Colasanti; Fabrizio Conti; Rachele Riganò; Elisabetta Profumo; Alessandra Siracusano; Antonella Capozzi; Massimiliano Prencipe; Maurizio Sorice; Ada Francia; Elena Ortona

doi:10.1016/j.jneuroim.2007.09.027

Screening of a microvascular endothelial cDNA library identifies rabaptin 5 as a novel autoantigen in Alzheimer's disease

Federica Delunardo, Paola Margutti, Simona Pontecorvo, Tania Colasanti, Fabrizio Conti, Rachele Riganò, Elisabetta Profumo, Alessandra Siracusano, Antonella Capozzi, Massimiliano Prencipe, Maurizio Sorice, Ada Francia, Elena Ortona

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Abstract

The pathogenesis of Alzheimer's disease (AD) includes the participation of the immune system. To identify antigenic targets in AD, we screened a human microvascular endothelial cell cDNA library with sera from patients with AD, and we identified rabaptin 5 (RABPT5). We detected serum IgG specific to RABPT5 in 65% of patients with AD and in 35% of patients with systemic lupus erythematosus, but in no healthy controls. Our results demonstrated a massive redistribution of this protein in the cytoplasm of endothelial and neuronal cells in apoptosis. In conclusion, we identified RABPT5 as a novel autoantigen in AD.

Original language	English
Pages (from-to)	105-112
Number of pages	8
Journal	Journal of Neuroimmunology
Volume	192
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2007.09.027
Publication status	Published - Dec 2007

Keywords

Alzheimer's disease
Autoantibodies
Endothelial cells
Systemic lupus erythematosus

ASJC Scopus subject areas

Immunology
Immunology and Allergy
Clinical Neurology
Neurology

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10.1016/j.jneuroim.2007.09.027

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Delunardo, F., Margutti, P., Pontecorvo, S., Colasanti, T., Conti, F., Riganò, R., Profumo, E., Siracusano, A., Capozzi, A., Prencipe, M., Sorice, M., Francia, A., & Ortona, E. (2007). Screening of a microvascular endothelial cDNA library identifies rabaptin 5 as a novel autoantigen in Alzheimer's disease. Journal of Neuroimmunology, 192(1-2), 105-112. https://doi.org/10.1016/j.jneuroim.2007.09.027

Screening of a microvascular endothelial cDNA library identifies rabaptin 5 as a novel autoantigen in Alzheimer's disease. / Delunardo, Federica; Margutti, Paola; Pontecorvo, Simona; Colasanti, Tania; Conti, Fabrizio; Riganò, Rachele; Profumo, Elisabetta; Siracusano, Alessandra; Capozzi, Antonella; Prencipe, Massimiliano; Sorice, Maurizio; Francia, Ada; Ortona, Elena.

In: Journal of Neuroimmunology, Vol. 192, No. 1-2, 12.2007, p. 105-112.

Research output: Contribution to journal › Article › peer-review

Delunardo, F, Margutti, P, Pontecorvo, S, Colasanti, T, Conti, F, Riganò, R, Profumo, E, Siracusano, A, Capozzi, A, Prencipe, M, Sorice, M, Francia, A & Ortona, E 2007, 'Screening of a microvascular endothelial cDNA library identifies rabaptin 5 as a novel autoantigen in Alzheimer's disease', Journal of Neuroimmunology, vol. 192, no. 1-2, pp. 105-112. https://doi.org/10.1016/j.jneuroim.2007.09.027

Delunardo, Federica ; Margutti, Paola ; Pontecorvo, Simona ; Colasanti, Tania ; Conti, Fabrizio ; Riganò, Rachele ; Profumo, Elisabetta ; Siracusano, Alessandra ; Capozzi, Antonella ; Prencipe, Massimiliano ; Sorice, Maurizio ; Francia, Ada ; Ortona, Elena. / Screening of a microvascular endothelial cDNA library identifies rabaptin 5 as a novel autoantigen in Alzheimer's disease. In: Journal of Neuroimmunology. 2007 ; Vol. 192, No. 1-2. pp. 105-112.

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abstract = "The pathogenesis of Alzheimer's disease (AD) includes the participation of the immune system. To identify antigenic targets in AD, we screened a human microvascular endothelial cell cDNA library with sera from patients with AD, and we identified rabaptin 5 (RABPT5). We detected serum IgG specific to RABPT5 in 65% of patients with AD and in 35% of patients with systemic lupus erythematosus, but in no healthy controls. Our results demonstrated a massive redistribution of this protein in the cytoplasm of endothelial and neuronal cells in apoptosis. In conclusion, we identified RABPT5 as a novel autoantigen in AD.",

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AU - Conti, Fabrizio

AU - Riganò, Rachele

AU - Profumo, Elisabetta

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AU - Capozzi, Antonella

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AB - The pathogenesis of Alzheimer's disease (AD) includes the participation of the immune system. To identify antigenic targets in AD, we screened a human microvascular endothelial cell cDNA library with sera from patients with AD, and we identified rabaptin 5 (RABPT5). We detected serum IgG specific to RABPT5 in 65% of patients with AD and in 35% of patients with systemic lupus erythematosus, but in no healthy controls. Our results demonstrated a massive redistribution of this protein in the cytoplasm of endothelial and neuronal cells in apoptosis. In conclusion, we identified RABPT5 as a novel autoantigen in AD.

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Screening of a microvascular endothelial cDNA library identifies rabaptin 5 as a novel autoantigen in Alzheimer's disease

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Keywords

ASJC Scopus subject areas

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