SDF-1α-mediated modulation of synaptic transmission in rat cerebellum

Cristina Limatola, Aldo Giovannelli, Laura Maggi, Davide Ragozzino, Loriana Castellani, Maria Teresa Ciotti, Fabrizio Vacca, Delio Mercanti, Angela Santoni, Fabrizio Eusebi

Research output: Contribution to journalArticle

Abstract

The functional expression of the seven-transmembrane domain G protein-coupled chemokine receptor CXCR-4/fusin in rat nerve cell was demonstrated by staining with a polyclonal anti-CXCR-4 Ab, and by evaluating the calcium responses to the physiological agonist stromal-derived cell factor-1α (SDF-1α) in both cerebellar granule cells in culture and Purkinje neurons (PNs) in cerebellar slices. Cerebellar glial, granule and Purkinje cells showed a pronounced staining for CXCR-4. Furthermore, cultured granule cells exhibited Ca2+ transients elicited by the application of SDF-1α, both in cell bodies and in neuronal processes. Whole-cell patch-clamped PNs in cerebellar slices responded to SDF-1α application by a slow inward current followed by an increase of both intracellular Ca2+ level and spontaneous synaptic activity. In particular, the SDF-1α-induced slow inward current was considerably reduced by ionotropic glutamate receptor blockers, but developed fully in a medium in which synaptic transmission was inhibited, indicating that this current might be, at least in part, mediated by extrasynaptic glutamate, possibly released from the surrounding glial and/or nerve cells. Taken together, these findings indicate a functional involvement of CXCR-4 in the modulation of synaptic transmission, adding another member to the repertoire of the chemokine receptors exerting a neuromodulatory role in the cerebellum.

Original languageEnglish
Pages (from-to)2497-2504
Number of pages8
JournalEuropean Journal of Neuroscience
Volume12
Issue number7
DOIs
Publication statusPublished - 2000

Keywords

  • Cerebellar granule cells
  • Cerebellar slice
  • Chemokine receptors
  • CXCR-4
  • Glutamatergic neurotransmission
  • Purkinje neurons
  • SDF-1α

ASJC Scopus subject areas

  • Neuroscience(all)

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  • Cite this

    Limatola, C., Giovannelli, A., Maggi, L., Ragozzino, D., Castellani, L., Ciotti, M. T., Vacca, F., Mercanti, D., Santoni, A., & Eusebi, F. (2000). SDF-1α-mediated modulation of synaptic transmission in rat cerebellum. European Journal of Neuroscience, 12(7), 2497-2504. https://doi.org/10.1046/j.1460-9568.2000.00139.x