Sea urchin neural α2 tubulin gene: Isolation and promoter analysis

S. Costa, M. A. Ragusa, G. Drago, C. Casano, G. Alaimo, N. Guida, F. Gianguzza

Research output: Contribution to journalArticle

Abstract

Expression of Tα2 gene, during sea urchin Paracentrotus lividus development, is spatially and temporally regulated. In order to characterize this gene, we isolated the relevant genomic sequences and scanned the isolated 5′-flanking region in searching for cis-regulatory elements required for proper expression. Gel mobility shift and footprinting assays, as well as reporter gene (CAT and β-gal) expression assays, were used to address cis-regulatory elements involved in regulation. Here we report that an upstream 5′-flanking fragment of PlTα2 gene drives temporal expression of reporter genes congruent with that of endogenous Tα2 gene. The fragment contains cis-elements able to bind nuclear proteins from the gastrula stage (at which the Tα2 gene is expressed) whose sequences could be consistent with the consensus sequences for transcription factors present in data bank.

Original languageEnglish
Pages (from-to)446-453
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume316
Issue number2
DOIs
Publication statusPublished - Apr 2 2004

Keywords

  • Ectopic expression
  • Neurogenesis
  • Paracentrotus lividus
  • Promoter
  • Tubulin genes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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