Searching the point of no return in Helicobacter pylori life: Necrosis and/or programmed death?

L. Cellini, I. Robuffo, N. M. Maraldi, G. Donelli

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Aims: Ultrastructural and molecular studies to support the hypothesis of programmed cell death in Helicobacter pylori were conducted. Methods and Results: Evidence of programmed death in H. pylori is provided through electron microscopic detection and cytochemical labelling of electrondense bodies (EDB), containing packaged DNA in coccoid cells, resembling micronuclei of apoptotic eukaryotic cells. This morphological evidence is also supported by DNA cleavage in homogeneous fragments of about 100 base pairs. Programmed cell death was observed in H. pylori cultures at 37 °C, with a maximum of 37.5% of EDB coccoid cells after 7 days. The non-permissive temperature of 4 °C anticipated this process, with 40% of EDB coccoid forms within 3 days, and it remained substantially unaffected during the observation time of 14 days. Conclusion: In these experiments, deprivation of nutrients and a non-permissive temperature acted as a powerful trigger for programmed cell death. Significance and Impact of the Study: Helicobacter pylori bacterial populations, under stressing stimuli, can respond with programmed cell suicide as a means of species preservation.

Original languageEnglish
Pages (from-to)727-732
Number of pages6
JournalJournal of Applied Microbiology
Volume90
Issue number5
DOIs
Publication statusPublished - 2001

Fingerprint

Helicobacter pylori
necrosis
Necrosis
death
Cell Death
apoptosis
DNA Cleavage
suicide
Temperature
Eukaryotic Cells
DNA
cells
Base Pairing
Suicide
eukaryotic cells
temperature
electrons
Observation
Electrons
Food

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Microbiology

Cite this

Searching the point of no return in Helicobacter pylori life : Necrosis and/or programmed death? / Cellini, L.; Robuffo, I.; Maraldi, N. M.; Donelli, G.

In: Journal of Applied Microbiology, Vol. 90, No. 5, 2001, p. 727-732.

Research output: Contribution to journalArticle

Cellini, L. ; Robuffo, I. ; Maraldi, N. M. ; Donelli, G. / Searching the point of no return in Helicobacter pylori life : Necrosis and/or programmed death?. In: Journal of Applied Microbiology. 2001 ; Vol. 90, No. 5. pp. 727-732.
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