Abstract
Aims: Ultrastructural and molecular studies to support the hypothesis of programmed cell death in Helicobacter pylori were conducted. Methods and Results: Evidence of programmed death in H. pylori is provided through electron microscopic detection and cytochemical labelling of electrondense bodies (EDB), containing packaged DNA in coccoid cells, resembling micronuclei of apoptotic eukaryotic cells. This morphological evidence is also supported by DNA cleavage in homogeneous fragments of about 100 base pairs. Programmed cell death was observed in H. pylori cultures at 37 °C, with a maximum of 37.5% of EDB coccoid cells after 7 days. The non-permissive temperature of 4 °C anticipated this process, with 40% of EDB coccoid forms within 3 days, and it remained substantially unaffected during the observation time of 14 days. Conclusion: In these experiments, deprivation of nutrients and a non-permissive temperature acted as a powerful trigger for programmed cell death. Significance and Impact of the Study: Helicobacter pylori bacterial populations, under stressing stimuli, can respond with programmed cell suicide as a means of species preservation.
| Original language | English |
|---|---|
| Pages (from-to) | 727-732 |
| Number of pages | 6 |
| Journal | Journal of Applied Microbiology |
| Volume | 90 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2001 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Applied Microbiology and Biotechnology
- Biotechnology
- Microbiology
Cite this
Searching the point of no return in Helicobacter pylori life : Necrosis and/or programmed death? / Cellini, L.; Robuffo, I.; Maraldi, N. M.; Donelli, G.
In: Journal of Applied Microbiology, Vol. 90, No. 5, 2001, p. 727-732.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Searching the point of no return in Helicobacter pylori life
T2 - Necrosis and/or programmed death?
AU - Cellini, L.
AU - Robuffo, I.
AU - Maraldi, N. M.
AU - Donelli, G.
PY - 2001
Y1 - 2001
N2 - Aims: Ultrastructural and molecular studies to support the hypothesis of programmed cell death in Helicobacter pylori were conducted. Methods and Results: Evidence of programmed death in H. pylori is provided through electron microscopic detection and cytochemical labelling of electrondense bodies (EDB), containing packaged DNA in coccoid cells, resembling micronuclei of apoptotic eukaryotic cells. This morphological evidence is also supported by DNA cleavage in homogeneous fragments of about 100 base pairs. Programmed cell death was observed in H. pylori cultures at 37 °C, with a maximum of 37.5% of EDB coccoid cells after 7 days. The non-permissive temperature of 4 °C anticipated this process, with 40% of EDB coccoid forms within 3 days, and it remained substantially unaffected during the observation time of 14 days. Conclusion: In these experiments, deprivation of nutrients and a non-permissive temperature acted as a powerful trigger for programmed cell death. Significance and Impact of the Study: Helicobacter pylori bacterial populations, under stressing stimuli, can respond with programmed cell suicide as a means of species preservation.
AB - Aims: Ultrastructural and molecular studies to support the hypothesis of programmed cell death in Helicobacter pylori were conducted. Methods and Results: Evidence of programmed death in H. pylori is provided through electron microscopic detection and cytochemical labelling of electrondense bodies (EDB), containing packaged DNA in coccoid cells, resembling micronuclei of apoptotic eukaryotic cells. This morphological evidence is also supported by DNA cleavage in homogeneous fragments of about 100 base pairs. Programmed cell death was observed in H. pylori cultures at 37 °C, with a maximum of 37.5% of EDB coccoid cells after 7 days. The non-permissive temperature of 4 °C anticipated this process, with 40% of EDB coccoid forms within 3 days, and it remained substantially unaffected during the observation time of 14 days. Conclusion: In these experiments, deprivation of nutrients and a non-permissive temperature acted as a powerful trigger for programmed cell death. Significance and Impact of the Study: Helicobacter pylori bacterial populations, under stressing stimuli, can respond with programmed cell suicide as a means of species preservation.
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UR - http://www.scopus.com/inward/citedby.url?scp=0035037498&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2672.2001.01300.x
DO - 10.1046/j.1365-2672.2001.01300.x
M3 - Article
C2 - 11348432
AN - SCOPUS:0035037498
VL - 90
SP - 727
EP - 732
JO - Journal of Applied Microbiology
JF - Journal of Applied Microbiology
SN - 1364-5072
IS - 5
ER -