Seasonal effect on airborne pyrene, urinary 1-hydroxypyrene, and benzo(a)pyrene diol epoxide-hemoglobin adducts in the general population

Roberta Pastorelli, Marco Guanci, Jolanda Restano, Angelo Berri, Giuseppina Micoli, Claudio Minoia, Daniela Alcini, Paolo Carrer, Eva Negri, Carlo La Vecchia, Roberto Fanelli, Luisa Airoldi

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Abstract

Exposure to airborne polycyclic aromatic hydrocarbons (PAHs) in 65 employees (40 sampled both in summer and winter, 15 sampled in summer only, and 10 sampled in winter only) with no occupational exposure to PAHs was assessed by measuring: personal exposure to pyrene, urinary excretion of 1- hydroxypyrene (1-OHP), and benzo(a)pyrene diol epoxide adducts to hemoglobin (BPDE-Hb). Overall, office employees were exposed to significantly higher levels of pyrene in winter (4.54 ± 2.35 ng/m3, mean ± SD) than in summer (1.67 ± 1.92 ng/m3, mean ± SD; P <0.001), but no such seasonal variability was observed in 1-OHP excretion. Tobacco smoking was the major determinant of 1-OHP excretion. BPDE-Hb adducts were measured by gas chromatography-mass spectrometry as benzo(a)pyrene tetrols (BPT) released from adducted hemoglobin. In the 65 employees analyzed, mean BPT levels ± SD were higher in winter (0.14 ± 0.38 fmol/mg Hb) than summer (0.031 ± 0.022 fmol/mg Hb). This difference was not statistically significant, probably because of the small proportion of subjects with detectable adducts (11% in summer and 16% in winter). BPDE-Hb adducts were not significantly associated with sex, age, diet, smoking habits, or with pyrene levels and 1-OHP excretion. This is the first report providing reference BPDE-Hb adduct values for the general population not occupationally exposed to environmental PAHs and shows a tendency to seasonal variability, with higher BPT levels in winter when environmental PAHs are also high.

Original languageEnglish
Pages (from-to)561-565
Number of pages5
JournalCancer Epidemiology Biomarkers and Prevention
Volume8
Issue number6
Publication statusPublished - Jun 1999

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Benzo(a)pyrene
Epoxy Compounds
Polycyclic Aromatic Hydrocarbons
Hemoglobins
Population
Smoking
Feeding Behavior
Occupational Exposure
Gas Chromatography-Mass Spectrometry
1-hydroxypyrene
pyrene

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Cite this

Seasonal effect on airborne pyrene, urinary 1-hydroxypyrene, and benzo(a)pyrene diol epoxide-hemoglobin adducts in the general population. / Pastorelli, Roberta; Guanci, Marco; Restano, Jolanda; Berri, Angelo; Micoli, Giuseppina; Minoia, Claudio; Alcini, Daniela; Carrer, Paolo; Negri, Eva; La Vecchia, Carlo; Fanelli, Roberto; Airoldi, Luisa.

In: Cancer Epidemiology Biomarkers and Prevention, Vol. 8, No. 6, 06.1999, p. 561-565.

Research output: Contribution to journalArticle

Pastorelli, Roberta ; Guanci, Marco ; Restano, Jolanda ; Berri, Angelo ; Micoli, Giuseppina ; Minoia, Claudio ; Alcini, Daniela ; Carrer, Paolo ; Negri, Eva ; La Vecchia, Carlo ; Fanelli, Roberto ; Airoldi, Luisa. / Seasonal effect on airborne pyrene, urinary 1-hydroxypyrene, and benzo(a)pyrene diol epoxide-hemoglobin adducts in the general population. In: Cancer Epidemiology Biomarkers and Prevention. 1999 ; Vol. 8, No. 6. pp. 561-565.
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abstract = "Exposure to airborne polycyclic aromatic hydrocarbons (PAHs) in 65 employees (40 sampled both in summer and winter, 15 sampled in summer only, and 10 sampled in winter only) with no occupational exposure to PAHs was assessed by measuring: personal exposure to pyrene, urinary excretion of 1- hydroxypyrene (1-OHP), and benzo(a)pyrene diol epoxide adducts to hemoglobin (BPDE-Hb). Overall, office employees were exposed to significantly higher levels of pyrene in winter (4.54 ± 2.35 ng/m3, mean ± SD) than in summer (1.67 ± 1.92 ng/m3, mean ± SD; P <0.001), but no such seasonal variability was observed in 1-OHP excretion. Tobacco smoking was the major determinant of 1-OHP excretion. BPDE-Hb adducts were measured by gas chromatography-mass spectrometry as benzo(a)pyrene tetrols (BPT) released from adducted hemoglobin. In the 65 employees analyzed, mean BPT levels ± SD were higher in winter (0.14 ± 0.38 fmol/mg Hb) than summer (0.031 ± 0.022 fmol/mg Hb). This difference was not statistically significant, probably because of the small proportion of subjects with detectable adducts (11{\%} in summer and 16{\%} in winter). BPDE-Hb adducts were not significantly associated with sex, age, diet, smoking habits, or with pyrene levels and 1-OHP excretion. This is the first report providing reference BPDE-Hb adduct values for the general population not occupationally exposed to environmental PAHs and shows a tendency to seasonal variability, with higher BPT levels in winter when environmental PAHs are also high.",
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AU - Restano, Jolanda

AU - Berri, Angelo

AU - Micoli, Giuseppina

AU - Minoia, Claudio

AU - Alcini, Daniela

AU - Carrer, Paolo

AU - Negri, Eva

AU - La Vecchia, Carlo

AU - Fanelli, Roberto

AU - Airoldi, Luisa

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