Secretion of IFN-γ, IL-6, granulocyte-macrophage colony-stimulating factor and IL-10 cytokines after activation of human purified T lymphocytes upon CD38 ligation

Clara M. Ausiello, Andrea La Sala, Carlo Ramoni, Francesca Urbani, Ada Funaro, Fabio Malavasi

Research output: Contribution to journalArticlepeer-review

Abstract

Human CD38, a surface glycoprotein expressed by different immunocompetent cells, is associated with distinct transmembrane signaling molecules and plays a key role in the synthesis of cyclic ABP-ribose, a calcium-mobilizing compound. This study reports that CD38 ligation by specific monoclonal antibodies (mAb) in purified peripheral blood T cells is followed by secretion of discrete cytokines. IL-6, granulocyte-macrophage colony-stimulating factor (GM-CSF), IFN-γ, and IL-10 mRNA expression were constant findings. Low levels of IL-2 mRNA were also detected in CD38-activated T-lymphocyte cultures of all subjects studied. Low levels of IL-4 and IL-5 mRNA were detected in the majority of CD38-activated T cultures. Moreover, CD38 mediated cytokine induction does not require T cell proliferation or the addition of antigen presenting cells. In conclusion, human CD38 runs an activation pathway in purified T cells which operates through the induction of a cytokine profile shared by Th1 or Th2 cells.

Original languageEnglish
Pages (from-to)192-197
Number of pages6
JournalCellular Immunology
Volume173
Issue number2
DOIs
Publication statusPublished - Nov 1 1996

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

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