Secretome compartment is a valuable source of biomarkers for cancer-relevant pathways

Dario Caccia, Laura Zanetti Domingues, Francesca Miccichè, Maida De Bortoli, Cristiana Carniti, Piera Mondellini, Italia Bongarzone

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

In principle, targeted therapies have optimal activity against a specific subset of tumors that depend upon the targeted molecule or pathway for growth, survival, or metastasis. Consequently, it is important in drug development and clinical practice to have predictive biomarkers that can reliably identify patients who will benefit from a given therapy. We analyzed tumor cell-line secretomes (conditioned cell media) to look for predictive biomarkers; secretomes represent a potential source for potential biomarkers that are expressed in intracellular signaling and therefore may reflect changes induced by targeted therapy. Using Gene Ontology, we classified by function the secretome proteins of 12 tumor cell lines of different histotypes. Representations and hierarchical relationships among the functional groups differed among the cell lines. Using bioinformatics tools, we identified proteins involved in intracellular signaling pathways. For example, we found that secretome proteins related to TGF-beta signaling in thyroid cancer cells, such as vasorin, CD109, and βIG-H3 (TGFBI), were sensitive to RPI-1 and dasatinib treatments, which have been previously demonstrated to be effective in blocking cell proliferation. The secretome may be a valuable source of potential biomarkers for detecting cancer and measuring the effectiveness of cancer therapies.

Original languageEnglish
Pages (from-to)4196-4207
Number of pages12
JournalJournal of Proteome Research
Volume10
Issue number9
DOIs
Publication statusPublished - Sep 2 2011

Fingerprint

Tumor Biomarkers
Biomarkers
Cells
Tumors
Tumor Cell Line
Proteins
Cell proliferation
Therapeutics
Bioinformatics
Gene Ontology
Transforming Growth Factor beta
Functional groups
Ontology
Conditioned Culture Medium
Computational Biology
Thyroid Neoplasms
Genes
Neoplasms
Molecules
Cell Proliferation

Keywords

  • adhesion pathway
  • bioinformatics
  • network prediction
  • predictive biomarkers
  • proteomics
  • secretome
  • signaling pathways
  • targeted therapy

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Secretome compartment is a valuable source of biomarkers for cancer-relevant pathways. / Caccia, Dario; Zanetti Domingues, Laura; Miccichè, Francesca; De Bortoli, Maida; Carniti, Cristiana; Mondellini, Piera; Bongarzone, Italia.

In: Journal of Proteome Research, Vol. 10, No. 9, 02.09.2011, p. 4196-4207.

Research output: Contribution to journalArticle

Caccia, D, Zanetti Domingues, L, Miccichè, F, De Bortoli, M, Carniti, C, Mondellini, P & Bongarzone, I 2011, 'Secretome compartment is a valuable source of biomarkers for cancer-relevant pathways', Journal of Proteome Research, vol. 10, no. 9, pp. 4196-4207. https://doi.org/10.1021/pr200344n
Caccia D, Zanetti Domingues L, Miccichè F, De Bortoli M, Carniti C, Mondellini P et al. Secretome compartment is a valuable source of biomarkers for cancer-relevant pathways. Journal of Proteome Research. 2011 Sep 2;10(9):4196-4207. https://doi.org/10.1021/pr200344n
Caccia, Dario ; Zanetti Domingues, Laura ; Miccichè, Francesca ; De Bortoli, Maida ; Carniti, Cristiana ; Mondellini, Piera ; Bongarzone, Italia. / Secretome compartment is a valuable source of biomarkers for cancer-relevant pathways. In: Journal of Proteome Research. 2011 ; Vol. 10, No. 9. pp. 4196-4207.
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