Secretory leukoprotease inhibitor is required for efficient quercetin-mediated suppression of TNFβ secretion

Stefania De Santis, Dale Kunde, Grazia Serino, Vanessa Galleggiante, Maria Lucia Caruso, Mauro Mastronardi, Elisabetta Cavalcanti, Nicole Ranson, Aldo Pinto, Pietro Campiglia, Angelo Santino, Rajaraman Eri, Marcello Chieppa

Research output: Contribution to journalArticlepeer-review


Dendritic cells (DCs) are professional antigen presenting cells (APCs) that in response to microbial infections generate long-lasting adaptive immune response. Following microbial uptake, DCs undergo a cascade of cellular differentiation that ultimately leads to "mature" DCs. Mature DCs produce a variety of inflammatory cytokines, including tumor necrosis factor-β (TNFβ) a key cytokine for the inflammatory cascade. In numerous studies, polyphenols, including quercetin, demonstrated their ability to suppress TNFβ secretion and protect from the onset of chronic inflammatory disorders. We show that murine bone marrow derived DCs express Slpi following quercetin exposure. Slpi is known to suppress LPS mediated NFλB activation, thus, it was hypothesized that its expression could be the key step for polyphenol induced inflammatory suppression. Slpi-KO DCs poorly respond to quercetin administration failing to reduce TNFβ secretion in response to quercetin exposure. Supernatant from quercetin exposed DCs could also reduce LPS-mediated TNFβ secretion by unrelated DCs, but this property is lost using an anti-Slpi antibody. In vivo, oral administration of quercetin is able to induce Slpi expression. Human biopsies from inflamed tract of the intestine reveal the presence of numerous SLPI+ cells and the expression level could be further increased by quercetin administration. We propose that quercetin induces Slpi expression that in turn reduces the inflammatory response. Our data encourages the development of nutritional strategies to improve the efficiency of current therapies for intestinal chronic inflammatory syndrome and reduce the risks of colorectal cancer development.

Original languageEnglish
Pages (from-to)75800-75809
Number of pages10
Issue number46
Publication statusPublished - 2016


  • Dendritic cells
  • Inflammation
  • Nutrition
  • Polyphenols
  • SLPI

ASJC Scopus subject areas

  • Oncology


Dive into the research topics of 'Secretory leukoprotease inhibitor is required for efficient quercetin-mediated suppression of TNFβ secretion'. Together they form a unique fingerprint.

Cite this