Segregation of type 1 cytokine production in human peripheral blood lymphocytes: Phenotypic differences between IFN-γ and IL-2-producing cells in the CD8+ T cell subset

Arnaldo Caruso, Stefano Licenziati, Daniele Morelli, Simona Fiorentini, Doris Ricotta, Fabio Malacarne, Lucia Sfondrini, Andrea Balsari

Research output: Contribution to journalArticle

Abstract

T cell clones are classified as type 0, 1 or 2 depending on the lymphokines they produce. However, it has remained unclear whether single cells of a given type produce one or several cytokine species. Flow cytometric analysis of peripheral blood lymphocytes (PBL) obtained from 20 healthy donors for the production of the type 1 cytokines IFN-γ and IL-2 revealed very few cells that co-expressed both cytokines independently of the mitogenic stimulus used for PBL activation. Similarly, kinetic studies of cytokine synthesis indicated a low per centage of IFN-γ/IL-2 double-positive T cells at all time points. Reverse transcription-PCR analysis of sorted IL-2- and IFN-γ-positive T cells showed the presence of IL-2- or IFN-γ-specific mRNA only in those cells expressing the corresponding cytokine. This segregation of the two type 1 cytokines was lost in long-term cultured T cells and in T cell clones. A high percentage of cells expressing only IL-2 or IFN-γ was observed even when the production of these cytokines was evaluated on CD4+ and CD8+ subsets. Moreover, in some healthy individuals, IFN-γ and IL-2 production by CD8+ T cells was related to CD8+ expression levels and cell size, i.e. IL-2-expressing cells were generally smaller with more intense CD8+ staining as compared with IFN-γ-producing T cells. These data indicate that activated T lymphocytes are strongly committed in vivo to produce IFN-γ or IL-2 and emphasizes the independent regulation of the two cytokine genes.

Original languageEnglish
Pages (from-to)3630-3638
Number of pages9
JournalEuropean Journal of Immunology
Volume28
Issue number11
DOIs
Publication statusPublished - Nov 1998

Fingerprint

T-Lymphocyte Subsets
Interleukin-2
Lymphocytes
Cytokines
T-Lymphocytes
Clone Cells
Lymphokines
Lymphocyte Activation
Cell Size
Reverse Transcription
Cultured Cells
Staining and Labeling
Polymerase Chain Reaction
Messenger RNA
Genes

Keywords

  • CD8
  • Flow cytometry
  • IFN-γ
  • IL-2

ASJC Scopus subject areas

  • Immunology

Cite this

Segregation of type 1 cytokine production in human peripheral blood lymphocytes : Phenotypic differences between IFN-γ and IL-2-producing cells in the CD8+ T cell subset. / Caruso, Arnaldo; Licenziati, Stefano; Morelli, Daniele; Fiorentini, Simona; Ricotta, Doris; Malacarne, Fabio; Sfondrini, Lucia; Balsari, Andrea.

In: European Journal of Immunology, Vol. 28, No. 11, 11.1998, p. 3630-3638.

Research output: Contribution to journalArticle

Caruso, Arnaldo ; Licenziati, Stefano ; Morelli, Daniele ; Fiorentini, Simona ; Ricotta, Doris ; Malacarne, Fabio ; Sfondrini, Lucia ; Balsari, Andrea. / Segregation of type 1 cytokine production in human peripheral blood lymphocytes : Phenotypic differences between IFN-γ and IL-2-producing cells in the CD8+ T cell subset. In: European Journal of Immunology. 1998 ; Vol. 28, No. 11. pp. 3630-3638.
@article{6bd4e968d2c546bfb690742bd4877f69,
title = "Segregation of type 1 cytokine production in human peripheral blood lymphocytes: Phenotypic differences between IFN-γ and IL-2-producing cells in the CD8+ T cell subset",
abstract = "T cell clones are classified as type 0, 1 or 2 depending on the lymphokines they produce. However, it has remained unclear whether single cells of a given type produce one or several cytokine species. Flow cytometric analysis of peripheral blood lymphocytes (PBL) obtained from 20 healthy donors for the production of the type 1 cytokines IFN-γ and IL-2 revealed very few cells that co-expressed both cytokines independently of the mitogenic stimulus used for PBL activation. Similarly, kinetic studies of cytokine synthesis indicated a low per centage of IFN-γ/IL-2 double-positive T cells at all time points. Reverse transcription-PCR analysis of sorted IL-2- and IFN-γ-positive T cells showed the presence of IL-2- or IFN-γ-specific mRNA only in those cells expressing the corresponding cytokine. This segregation of the two type 1 cytokines was lost in long-term cultured T cells and in T cell clones. A high percentage of cells expressing only IL-2 or IFN-γ was observed even when the production of these cytokines was evaluated on CD4+ and CD8+ subsets. Moreover, in some healthy individuals, IFN-γ and IL-2 production by CD8+ T cells was related to CD8+ expression levels and cell size, i.e. IL-2-expressing cells were generally smaller with more intense CD8+ staining as compared with IFN-γ-producing T cells. These data indicate that activated T lymphocytes are strongly committed in vivo to produce IFN-γ or IL-2 and emphasizes the independent regulation of the two cytokine genes.",
keywords = "CD8, Flow cytometry, IFN-γ, IL-2",
author = "Arnaldo Caruso and Stefano Licenziati and Daniele Morelli and Simona Fiorentini and Doris Ricotta and Fabio Malacarne and Lucia Sfondrini and Andrea Balsari",
year = "1998",
month = "11",
doi = "10.1002/(SICI)1521-4141(199811)28:11<3630::AID-IMMU3630>3.0.CO;2-6",
language = "English",
volume = "28",
pages = "3630--3638",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "wiley",
number = "11",

}

TY - JOUR

T1 - Segregation of type 1 cytokine production in human peripheral blood lymphocytes

T2 - Phenotypic differences between IFN-γ and IL-2-producing cells in the CD8+ T cell subset

AU - Caruso, Arnaldo

AU - Licenziati, Stefano

AU - Morelli, Daniele

AU - Fiorentini, Simona

AU - Ricotta, Doris

AU - Malacarne, Fabio

AU - Sfondrini, Lucia

AU - Balsari, Andrea

PY - 1998/11

Y1 - 1998/11

N2 - T cell clones are classified as type 0, 1 or 2 depending on the lymphokines they produce. However, it has remained unclear whether single cells of a given type produce one or several cytokine species. Flow cytometric analysis of peripheral blood lymphocytes (PBL) obtained from 20 healthy donors for the production of the type 1 cytokines IFN-γ and IL-2 revealed very few cells that co-expressed both cytokines independently of the mitogenic stimulus used for PBL activation. Similarly, kinetic studies of cytokine synthesis indicated a low per centage of IFN-γ/IL-2 double-positive T cells at all time points. Reverse transcription-PCR analysis of sorted IL-2- and IFN-γ-positive T cells showed the presence of IL-2- or IFN-γ-specific mRNA only in those cells expressing the corresponding cytokine. This segregation of the two type 1 cytokines was lost in long-term cultured T cells and in T cell clones. A high percentage of cells expressing only IL-2 or IFN-γ was observed even when the production of these cytokines was evaluated on CD4+ and CD8+ subsets. Moreover, in some healthy individuals, IFN-γ and IL-2 production by CD8+ T cells was related to CD8+ expression levels and cell size, i.e. IL-2-expressing cells were generally smaller with more intense CD8+ staining as compared with IFN-γ-producing T cells. These data indicate that activated T lymphocytes are strongly committed in vivo to produce IFN-γ or IL-2 and emphasizes the independent regulation of the two cytokine genes.

AB - T cell clones are classified as type 0, 1 or 2 depending on the lymphokines they produce. However, it has remained unclear whether single cells of a given type produce one or several cytokine species. Flow cytometric analysis of peripheral blood lymphocytes (PBL) obtained from 20 healthy donors for the production of the type 1 cytokines IFN-γ and IL-2 revealed very few cells that co-expressed both cytokines independently of the mitogenic stimulus used for PBL activation. Similarly, kinetic studies of cytokine synthesis indicated a low per centage of IFN-γ/IL-2 double-positive T cells at all time points. Reverse transcription-PCR analysis of sorted IL-2- and IFN-γ-positive T cells showed the presence of IL-2- or IFN-γ-specific mRNA only in those cells expressing the corresponding cytokine. This segregation of the two type 1 cytokines was lost in long-term cultured T cells and in T cell clones. A high percentage of cells expressing only IL-2 or IFN-γ was observed even when the production of these cytokines was evaluated on CD4+ and CD8+ subsets. Moreover, in some healthy individuals, IFN-γ and IL-2 production by CD8+ T cells was related to CD8+ expression levels and cell size, i.e. IL-2-expressing cells were generally smaller with more intense CD8+ staining as compared with IFN-γ-producing T cells. These data indicate that activated T lymphocytes are strongly committed in vivo to produce IFN-γ or IL-2 and emphasizes the independent regulation of the two cytokine genes.

KW - CD8

KW - Flow cytometry

KW - IFN-γ

KW - IL-2

UR - http://www.scopus.com/inward/record.url?scp=0031771132&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031771132&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1521-4141(199811)28:11<3630::AID-IMMU3630>3.0.CO;2-6

DO - 10.1002/(SICI)1521-4141(199811)28:11<3630::AID-IMMU3630>3.0.CO;2-6

M3 - Article

C2 - 9842905

AN - SCOPUS:0031771132

VL - 28

SP - 3630

EP - 3638

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 11

ER -