SEL1L and HRD1 are involved in the degradation of unassembled secretory Ig-μ chains

Monica Cattaneo, Mieko Otsu, Claudio Fagioli, Simone Martino, Lavinia Vittoria Lotti, Roberto Sitia, Ida Biunno

Research output: Contribution to journalArticle

Abstract

When expressed in the absence of light chains, secretory Ig-μ chains (μs) undergo endoplasmic reticulum associated degradation (ERAD). This process involves the recognition of terminally misfolded or unassembled molecules, their retro-translocation across the ER membrane and ubiquitination for degradation by cytosolic proteasomes. The molecular components of the ERAD pathway and their coordination remain largely unknown. Here we employed co-immunoprecipitation, silencing or over-expression assays to show that SEL1L and HRD1 are involved in the degradation of unassembled Ig-μs, but have minor effects on another substrate, TCR-α. SEL1L and HRD1 localize in the early secretory apparatus and are induced by ER stress and during B cell differentiation, concomitantly with the onset of massive IgM secretion. These findings reveal a role for SEL1L and HRD1 in IgM quality control.

Original languageEnglish
Pages (from-to)794-802
Number of pages9
JournalJournal of Cellular Physiology
Volume215
Issue number3
DOIs
Publication statusPublished - Jun 2008

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ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

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