Tumor necrosis factor (TNFα and TNFβ) and interleukin-1 (IL-1) are mediators of immunity and inflammation that induce different, but partially overlapping responses in human endothelial cells (HEC). We compared the effect of purified recombinant human TNFα, TNFβ and IL-1 on the production of platelet-activating factor (PAF) in HEC. After 30 - 60 min of treatment with TNFα or TNFβ, HEC produce and partially elease considerable amounts of PAF, which reach a maximum after 4 - 6 h. In HEC treated with IL-1 PAF production is detectable after 2 h and peaks at 8 - 12 h. More than twice as much PAF is produced in response to optimal concentrations of TNFα than in response to TNFβ or IL-1. However, PAF synthesis is stimulated by lower molar concentrations of IL-1 than TNFα and TNFβ. The ability to induce PAF synthesis in HEC seems to be restricted to these three cytokins, as shown by negative results obtained with other cytokines that activate HEC (interferons, granulocyte- and granulocyte-macrophage colony-stimulating factor, epithelial growth factor, fibroblast growth factor, transforming growth factor β), or participate in the inflammatory process (IL-6, platelet-derived growth factor).
|Journal||Journal of Lipid Mediators|
|Publication status||Published - 1990|
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