Selected pool of peptides from ESAT-6 and CFP-10 proteins for detection of Mycobacterium tuberculosis infection

Paolo Scarpellini, Silvana Tasca, Laura Galli, Alberto Beretta, Adriano Lazzarin, Claudio Fortis

Research output: Contribution to journalArticle

Abstract

We have validated a new test for detecting Mycobacterium tuberculosis infection. A pool of synthetic peptides derived from ESAT-6 and CFP-10 proteins was used to detect the number of specific gamma interferon-producing T cells by means of an enzyme-linked immunospot assay. Sixty-eight individuals positive for M. tuberculosis infection, either human immunodeficiency virus-seropositive or -seronegative, were studied. The test results were highly specific (87.5%) and sensitive (93.1%), more so than a classical lymphoproliferative assay (specificity and sensitivity of 77.27%), opening new possibilities for diagnosis and screening of tuberculosis. Moreover, the test allowed us to distinguish individuals infected with M. tuberculosis from those vaccinated with BCG.

Original languageEnglish
Pages (from-to)3469-3474
Number of pages6
JournalJournal of Clinical Microbiology
Volume42
Issue number8
DOIs
Publication statusPublished - Aug 2004

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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