Selection and fine mapping of chromosome-specific cDNAs: Application to human chromosome 1

Mita Mancini, Cinzia Sala, Stefano Rivella, Daniela Toniolo

Research output: Contribution to journalArticlepeer-review

Abstract

We have developed a methodology for identification and fine mapping of chromosome-specific transcripts. Combining digestion of DNA with different restriction enzymes, ligation to 'bubble' linkers, and PCR amplification from Alu and 'bubble' primers, we have synthesized human chromosome 1- specific sequences from DNA of a somatic cell hybrid, A9Neo1. After hybridization to human fetal brain cDNA, we could efficiently capture chromosome 1-specific cDNAs. The cDNAs were sequenced and used as probes in hybridizations to high-density filters containing the arrayed CEPH Mega-YAC library and to the arrayed cDNA library from infant brain made by B. Soares, which has been extensively sequenced. By this approach we have been able to select chromosome 1-specific cDNAs, to map them to chromosome 1 YAC contigs, and to identify and map corresponding longer cDNAs and ESTs.

Original languageEnglish
Pages (from-to)149-154
Number of pages6
JournalGenomics
Volume38
Issue number2
DOIs
Publication statusPublished - Dec 1 1996

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Selection and fine mapping of chromosome-specific cDNAs: Application to human chromosome 1'. Together they form a unique fingerprint.

Cite this