Selection and mapping of replication origins from a 500-kb region of the human X chromosome and their relationship to gene expression

Stefano Rivella, Belinda Palermo, Cristina Pelizon, Cinzia Sala, Giulia Arrigo, Daniela Toniolo

Research output: Contribution to journalArticle

Abstract

In higher eukaryotes the mechanism controlling initiation of DNA replication remains largely unknown. New technologies are needed to shed light on how DNA replication initiates along the genome in specific regions. To identify the human DNA sequence requirements for initiation of replication, we developed a new method that allows selection of replication origins starting from large genomic regions of human DNA. We repeatedly isolated 15 new putative replication origins (PROs) from a human DNA region of 500 kb in which 17 genes have previously been characterized. Fine-mapping of these PROs showed that DNA replication can initiate at many specific points along actively transcribed DNA in the cell lines used for our selection. In conclusion, in this paper we describe a new method to identify PROs that suggests that the availability of initiation sites is dependent on the transcriptional state of the DNA.

Original languageEnglish
Pages (from-to)11-20
Number of pages10
JournalGenomics
Volume62
Issue number1
DOIs
Publication statusPublished - Nov 15 1999

Fingerprint

Chromosomes, Human, X
Replication Origin
DNA Replication
Gene Expression
DNA
Eukaryota
Genome
Technology
Cell Line
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Selection and mapping of replication origins from a 500-kb region of the human X chromosome and their relationship to gene expression. / Rivella, Stefano; Palermo, Belinda; Pelizon, Cristina; Sala, Cinzia; Arrigo, Giulia; Toniolo, Daniela.

In: Genomics, Vol. 62, No. 1, 15.11.1999, p. 11-20.

Research output: Contribution to journalArticle

Rivella, Stefano ; Palermo, Belinda ; Pelizon, Cristina ; Sala, Cinzia ; Arrigo, Giulia ; Toniolo, Daniela. / Selection and mapping of replication origins from a 500-kb region of the human X chromosome and their relationship to gene expression. In: Genomics. 1999 ; Vol. 62, No. 1. pp. 11-20.
@article{b2b723a49272445b8e77a217d33dfac2,
title = "Selection and mapping of replication origins from a 500-kb region of the human X chromosome and their relationship to gene expression",
abstract = "In higher eukaryotes the mechanism controlling initiation of DNA replication remains largely unknown. New technologies are needed to shed light on how DNA replication initiates along the genome in specific regions. To identify the human DNA sequence requirements for initiation of replication, we developed a new method that allows selection of replication origins starting from large genomic regions of human DNA. We repeatedly isolated 15 new putative replication origins (PROs) from a human DNA region of 500 kb in which 17 genes have previously been characterized. Fine-mapping of these PROs showed that DNA replication can initiate at many specific points along actively transcribed DNA in the cell lines used for our selection. In conclusion, in this paper we describe a new method to identify PROs that suggests that the availability of initiation sites is dependent on the transcriptional state of the DNA.",
author = "Stefano Rivella and Belinda Palermo and Cristina Pelizon and Cinzia Sala and Giulia Arrigo and Daniela Toniolo",
year = "1999",
month = "11",
day = "15",
doi = "10.1006/geno.1999.5985",
language = "English",
volume = "62",
pages = "11--20",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Selection and mapping of replication origins from a 500-kb region of the human X chromosome and their relationship to gene expression

AU - Rivella, Stefano

AU - Palermo, Belinda

AU - Pelizon, Cristina

AU - Sala, Cinzia

AU - Arrigo, Giulia

AU - Toniolo, Daniela

PY - 1999/11/15

Y1 - 1999/11/15

N2 - In higher eukaryotes the mechanism controlling initiation of DNA replication remains largely unknown. New technologies are needed to shed light on how DNA replication initiates along the genome in specific regions. To identify the human DNA sequence requirements for initiation of replication, we developed a new method that allows selection of replication origins starting from large genomic regions of human DNA. We repeatedly isolated 15 new putative replication origins (PROs) from a human DNA region of 500 kb in which 17 genes have previously been characterized. Fine-mapping of these PROs showed that DNA replication can initiate at many specific points along actively transcribed DNA in the cell lines used for our selection. In conclusion, in this paper we describe a new method to identify PROs that suggests that the availability of initiation sites is dependent on the transcriptional state of the DNA.

AB - In higher eukaryotes the mechanism controlling initiation of DNA replication remains largely unknown. New technologies are needed to shed light on how DNA replication initiates along the genome in specific regions. To identify the human DNA sequence requirements for initiation of replication, we developed a new method that allows selection of replication origins starting from large genomic regions of human DNA. We repeatedly isolated 15 new putative replication origins (PROs) from a human DNA region of 500 kb in which 17 genes have previously been characterized. Fine-mapping of these PROs showed that DNA replication can initiate at many specific points along actively transcribed DNA in the cell lines used for our selection. In conclusion, in this paper we describe a new method to identify PROs that suggests that the availability of initiation sites is dependent on the transcriptional state of the DNA.

UR - http://www.scopus.com/inward/record.url?scp=0032760808&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032760808&partnerID=8YFLogxK

U2 - 10.1006/geno.1999.5985

DO - 10.1006/geno.1999.5985

M3 - Article

C2 - 10585763

AN - SCOPUS:0032760808

VL - 62

SP - 11

EP - 20

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 1

ER -