Selection of a human chromosome 21 enriched YAC sub-library using a chromosome-specific composite probe

Mark T. Ross, Dean Nižetić, Catherine Nguyen, Catherine Knights, Radost Vatcheva, Neil Burden, Christal Douglas, Günther Zehetner, David C. Ward, Antonio Baldini, Hans Lehrach

Research output: Contribution to journalArticle

Abstract

The subdivision of total genomic human yeast artificial chromosome (YAC) libraries into specific chromosome clone collections will greatly facilitate the construction of an integrated genetic, physical and transcriptional map of the genome. We report the isolation of 388 YAC clones from a human library with an average insert size of 620 kilobases (kb) by the hybridization of a composite chromosome 21 probe to a high-density array of YAC clones. Roughly half of these clones hybridize to chromosome 21 by fluorescence in situ hybridization. These clones represent a twofold coverage of the chromosome. The technique offers the potential of sub-dividing whole genomic YAC libraries into their chromosomal elements to produce high-resolution tools for genome mapping.

Original languageEnglish
Pages (from-to)284-290
Number of pages7
JournalNature Genetics
Volume1
Issue number4
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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