TY - JOUR
T1 - Selection of donors significantly improves pig islet isolation yield
AU - Socci, C.
AU - Ricordi, C.
AU - Davalli, A. M.
AU - Staudacher, C.
AU - Baro, P.
AU - Vertova, A.
AU - Freschi, M.
AU - Gavazzi, F.
AU - Braghi, S.
AU - Pozza, G.
AU - Di Carlo, V.
PY - 1990
Y1 - 1990
N2 - The purpose of the present study was to test the influence of donor age onislet isolation yield in swines. Large White pigs of 10 months (group 1, n = 10) and 2-3 years (group 2, n = 5) were used. A modification of the automated method for human islet isolation was used for pig islet separation. After ductal injection of a collagenase solution (Seromed, type C- 1000) the islets were separated through a continuous digestion process. In group 1 it was possible to obtain an average of 636,100 islets (volume = 476.1 mm3, insulin content = 101.4 Units). In group 2 the average total islet number was 799,800 representing a volume of 1190.6 mm3 (insulin content 210 Units). Total islet number was not significantly different in the two groups, however when islet volume and total insulin content were considered, older animals allowed a significantly higher yield (P
AB - The purpose of the present study was to test the influence of donor age onislet isolation yield in swines. Large White pigs of 10 months (group 1, n = 10) and 2-3 years (group 2, n = 5) were used. A modification of the automated method for human islet isolation was used for pig islet separation. After ductal injection of a collagenase solution (Seromed, type C- 1000) the islets were separated through a continuous digestion process. In group 1 it was possible to obtain an average of 636,100 islets (volume = 476.1 mm3, insulin content = 101.4 Units). In group 2 the average total islet number was 799,800 representing a volume of 1190.6 mm3 (insulin content 210 Units). Total islet number was not significantly different in the two groups, however when islet volume and total insulin content were considered, older animals allowed a significantly higher yield (P
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M3 - Article
C2 - 2088980
AN - SCOPUS:0025689417
VL - 25
SP - 32
EP - 34
JO - Hormone and Metabolic Research
JF - Hormone and Metabolic Research
SN - 0018-5043
IS - SUPPL.
ER -