Selection of donors significantly improves pig islet isolation yield

C. Socci, C. Ricordi, A. M. Davalli, C. Staudacher, P. Baro, A. Vertova, M. Freschi, F. Gavazzi, S. Braghi, G. Pozza, V. Di Carlo

Research output: Contribution to journalArticlepeer-review

Abstract

The purpose of the present study was to test the influence of donor age onislet isolation yield in swines. Large White pigs of 10 months (group 1, n = 10) and 2-3 years (group 2, n = 5) were used. A modification of the automated method for human islet isolation was used for pig islet separation. After ductal injection of a collagenase solution (Seromed, type C- 1000) the islets were separated through a continuous digestion process. In group 1 it was possible to obtain an average of 636,100 islets (volume = 476.1 mm3, insulin content = 101.4 Units). In group 2 the average total islet number was 799,800 representing a volume of 1190.6 mm3 (insulin content 210 Units). Total islet number was not significantly different in the two groups, however when islet volume and total insulin content were considered, older animals allowed a significantly higher yield (P

Original languageEnglish
Pages (from-to)32-34
Number of pages3
JournalHormone and Metabolic Research
Volume25
Issue numberSUPPL.
Publication statusPublished - 1990

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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