Selection of phage-displayed peptides mimicking type 1 diabetes-specific epitopes

Carmela Mennuni, Claudia Santini, Francesco Dotta, Loredana Farilla, Umberto Di Mario, Alessandra Fierabracci, Gianfranco Bottazzo, Riccardo Cortese, Alessandra Luzzago

Research output: Contribution to journalArticlepeer-review

Abstract

Phage display technology represents a powerful tool for the identification of peptides reacting with disease-related antibodies present inhuman sera. The application of this technology to type 1 diabetes could provide a set of novel reagents for diabetes prediction and could also lead to the identification of novel autoantigens or even of environmental factors possibly causing the disease. In the present study, sera of prediabetic and high risk individuals were used to select candidate peptide from phage-displayed random peptide libraries. Diabetes specific phage clones were then identified from these through screening and counter screening, using sera from diabetic and non-diabetic individuals. The results presented in this paper demonstrate the feasibility of this methodology to identify peptides reacting preferentially with antibodies present in the serum of diabetic patients.

Original languageEnglish
Pages (from-to)431-436
Number of pages6
JournalJournal of Autoimmunity
Volume9
Issue number3
DOIs
Publication statusPublished - Jun 1996

Keywords

  • Affinity selection
  • Epitopes
  • Page peptide libraries
  • Type 1 diabetes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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