TY - JOUR
T1 - Selection of phage-displayed peptides mimicking type 1 diabetes-specific epitopes
AU - Mennuni, Carmela
AU - Santini, Claudia
AU - Dotta, Francesco
AU - Farilla, Loredana
AU - Di Mario, Umberto
AU - Fierabracci, Alessandra
AU - Bottazzo, Gianfranco
AU - Cortese, Riccardo
AU - Luzzago, Alessandra
PY - 1996/6
Y1 - 1996/6
N2 - Phage display technology represents a powerful tool for the identification of peptides reacting with disease-related antibodies present inhuman sera. The application of this technology to type 1 diabetes could provide a set of novel reagents for diabetes prediction and could also lead to the identification of novel autoantigens or even of environmental factors possibly causing the disease. In the present study, sera of prediabetic and high risk individuals were used to select candidate peptide from phage-displayed random peptide libraries. Diabetes specific phage clones were then identified from these through screening and counter screening, using sera from diabetic and non-diabetic individuals. The results presented in this paper demonstrate the feasibility of this methodology to identify peptides reacting preferentially with antibodies present in the serum of diabetic patients.
AB - Phage display technology represents a powerful tool for the identification of peptides reacting with disease-related antibodies present inhuman sera. The application of this technology to type 1 diabetes could provide a set of novel reagents for diabetes prediction and could also lead to the identification of novel autoantigens or even of environmental factors possibly causing the disease. In the present study, sera of prediabetic and high risk individuals were used to select candidate peptide from phage-displayed random peptide libraries. Diabetes specific phage clones were then identified from these through screening and counter screening, using sera from diabetic and non-diabetic individuals. The results presented in this paper demonstrate the feasibility of this methodology to identify peptides reacting preferentially with antibodies present in the serum of diabetic patients.
KW - Affinity selection
KW - Epitopes
KW - Page peptide libraries
KW - Type 1 diabetes
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U2 - 10.1006/jaut.1996.0060
DO - 10.1006/jaut.1996.0060
M3 - Article
C2 - 8816983
AN - SCOPUS:0030158835
VL - 9
SP - 431
EP - 436
JO - Journal of Autoimmunity
JF - Journal of Autoimmunity
SN - 0896-8411
IS - 3
ER -