Selection of resistance of telithromycin against Haemophilus influenzae, Moraxella catarrhalis and streptococci in comparison with macrolides

Lorenzo Drago, Elena De Vecchi, Lucia Nicola, Alberto Colombo, Maria Rita Gismondo

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objective: The in vitro abilities of telithromycin, azithromycin and clarithromycin to select for resistance were compared by testing isolates of Haemophilus influenzae, Moraxella catarrhalis, Streptococcus pneumoniae and β-haemolytic streptococci. Methods: Five strains each of β-lactamase-positive and β-lactamase-negative H. influenzae, β-lactamase-positive and β-lactamase-negative M. catarrhalis, S. pneumoniae, β-haemolytic group A, group C and group G streptococci and three strains of β-lactamase-negative ampicillin-resistant H. influenzae were evaluated. Development of resistance was determined by multi-step and single-step methodologies. For multi-step studies, MIC values were determined after five serial passages on anti-biotic-gradient plates and after 10 serial passages on antibiotic-free plates. Acquisition of resistance was defined as an increase of ≥4-fold from the starting MIC. In single-step studies, the rate of spontaneous mutations was calculated after a passage on plates containing antibiotics at concentrations equal to the highest NCCLS breakpoints. Results: Azithromycin, clarithromycin and telithromycin gave a ≥4-fold increase in 20, 20 and 10 streptococcus strains, in 4, 5 and 0 H. influenzae strains and in 2, 7 and 4 M. catarrhalis strains, respectively. After 10 passages on antibiotic-free plates, 21/26 strains for azithromycin, 22/32 for clarithromycin and 1/14 for telithromycin maintained high MIC values. In single-step studies, the frequency of mutations was -10 for H. influenzae and M. catarrhalis for telithromycin, azithromycin and clarithromycin. Telithromycin induced mutations at a lower rate than azithromycin and clarithromycin in streptococcal strains. Conclusion: Telithromycin showed a very limited ability to select for resistance in respiratory pathogens compared with azithromycin and clarithromycin.

Original languageEnglish
Pages (from-to)542-545
Number of pages4
JournalJournal of Antimicrobial Chemotherapy
Volume54
Issue number2
DOIs
Publication statusPublished - Aug 2004

Fingerprint

Moraxella (Branhamella) catarrhalis
Azithromycin
Clarithromycin
Macrolides
Haemophilus influenzae
Streptococcus
Serial Passage
Mutation Rate
Anti-Bacterial Agents
Streptococcus pneumoniae
Ampicillin
telithromycin
Mutation

Keywords

  • Azithromycin
  • Clarithromycin
  • In vitro selection of resistance
  • Ketolides
  • Respiratory pathogens

ASJC Scopus subject areas

  • Pharmacology
  • Microbiology

Cite this

Selection of resistance of telithromycin against Haemophilus influenzae, Moraxella catarrhalis and streptococci in comparison with macrolides. / Drago, Lorenzo; De Vecchi, Elena; Nicola, Lucia; Colombo, Alberto; Gismondo, Maria Rita.

In: Journal of Antimicrobial Chemotherapy, Vol. 54, No. 2, 08.2004, p. 542-545.

Research output: Contribution to journalArticle

@article{ee49890118de42dd96ea09e64bc65fbe,
title = "Selection of resistance of telithromycin against Haemophilus influenzae, Moraxella catarrhalis and streptococci in comparison with macrolides",
abstract = "Objective: The in vitro abilities of telithromycin, azithromycin and clarithromycin to select for resistance were compared by testing isolates of Haemophilus influenzae, Moraxella catarrhalis, Streptococcus pneumoniae and β-haemolytic streptococci. Methods: Five strains each of β-lactamase-positive and β-lactamase-negative H. influenzae, β-lactamase-positive and β-lactamase-negative M. catarrhalis, S. pneumoniae, β-haemolytic group A, group C and group G streptococci and three strains of β-lactamase-negative ampicillin-resistant H. influenzae were evaluated. Development of resistance was determined by multi-step and single-step methodologies. For multi-step studies, MIC values were determined after five serial passages on anti-biotic-gradient plates and after 10 serial passages on antibiotic-free plates. Acquisition of resistance was defined as an increase of ≥4-fold from the starting MIC. In single-step studies, the rate of spontaneous mutations was calculated after a passage on plates containing antibiotics at concentrations equal to the highest NCCLS breakpoints. Results: Azithromycin, clarithromycin and telithromycin gave a ≥4-fold increase in 20, 20 and 10 streptococcus strains, in 4, 5 and 0 H. influenzae strains and in 2, 7 and 4 M. catarrhalis strains, respectively. After 10 passages on antibiotic-free plates, 21/26 strains for azithromycin, 22/32 for clarithromycin and 1/14 for telithromycin maintained high MIC values. In single-step studies, the frequency of mutations was -10 for H. influenzae and M. catarrhalis for telithromycin, azithromycin and clarithromycin. Telithromycin induced mutations at a lower rate than azithromycin and clarithromycin in streptococcal strains. Conclusion: Telithromycin showed a very limited ability to select for resistance in respiratory pathogens compared with azithromycin and clarithromycin.",
keywords = "Azithromycin, Clarithromycin, In vitro selection of resistance, Ketolides, Respiratory pathogens",
author = "Lorenzo Drago and {De Vecchi}, Elena and Lucia Nicola and Alberto Colombo and Gismondo, {Maria Rita}",
year = "2004",
month = "8",
doi = "10.1093/jac/dkh339",
language = "English",
volume = "54",
pages = "542--545",
journal = "Journal of Antimicrobial Chemotherapy",
issn = "0305-7453",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - Selection of resistance of telithromycin against Haemophilus influenzae, Moraxella catarrhalis and streptococci in comparison with macrolides

AU - Drago, Lorenzo

AU - De Vecchi, Elena

AU - Nicola, Lucia

AU - Colombo, Alberto

AU - Gismondo, Maria Rita

PY - 2004/8

Y1 - 2004/8

N2 - Objective: The in vitro abilities of telithromycin, azithromycin and clarithromycin to select for resistance were compared by testing isolates of Haemophilus influenzae, Moraxella catarrhalis, Streptococcus pneumoniae and β-haemolytic streptococci. Methods: Five strains each of β-lactamase-positive and β-lactamase-negative H. influenzae, β-lactamase-positive and β-lactamase-negative M. catarrhalis, S. pneumoniae, β-haemolytic group A, group C and group G streptococci and three strains of β-lactamase-negative ampicillin-resistant H. influenzae were evaluated. Development of resistance was determined by multi-step and single-step methodologies. For multi-step studies, MIC values were determined after five serial passages on anti-biotic-gradient plates and after 10 serial passages on antibiotic-free plates. Acquisition of resistance was defined as an increase of ≥4-fold from the starting MIC. In single-step studies, the rate of spontaneous mutations was calculated after a passage on plates containing antibiotics at concentrations equal to the highest NCCLS breakpoints. Results: Azithromycin, clarithromycin and telithromycin gave a ≥4-fold increase in 20, 20 and 10 streptococcus strains, in 4, 5 and 0 H. influenzae strains and in 2, 7 and 4 M. catarrhalis strains, respectively. After 10 passages on antibiotic-free plates, 21/26 strains for azithromycin, 22/32 for clarithromycin and 1/14 for telithromycin maintained high MIC values. In single-step studies, the frequency of mutations was -10 for H. influenzae and M. catarrhalis for telithromycin, azithromycin and clarithromycin. Telithromycin induced mutations at a lower rate than azithromycin and clarithromycin in streptococcal strains. Conclusion: Telithromycin showed a very limited ability to select for resistance in respiratory pathogens compared with azithromycin and clarithromycin.

AB - Objective: The in vitro abilities of telithromycin, azithromycin and clarithromycin to select for resistance were compared by testing isolates of Haemophilus influenzae, Moraxella catarrhalis, Streptococcus pneumoniae and β-haemolytic streptococci. Methods: Five strains each of β-lactamase-positive and β-lactamase-negative H. influenzae, β-lactamase-positive and β-lactamase-negative M. catarrhalis, S. pneumoniae, β-haemolytic group A, group C and group G streptococci and three strains of β-lactamase-negative ampicillin-resistant H. influenzae were evaluated. Development of resistance was determined by multi-step and single-step methodologies. For multi-step studies, MIC values were determined after five serial passages on anti-biotic-gradient plates and after 10 serial passages on antibiotic-free plates. Acquisition of resistance was defined as an increase of ≥4-fold from the starting MIC. In single-step studies, the rate of spontaneous mutations was calculated after a passage on plates containing antibiotics at concentrations equal to the highest NCCLS breakpoints. Results: Azithromycin, clarithromycin and telithromycin gave a ≥4-fold increase in 20, 20 and 10 streptococcus strains, in 4, 5 and 0 H. influenzae strains and in 2, 7 and 4 M. catarrhalis strains, respectively. After 10 passages on antibiotic-free plates, 21/26 strains for azithromycin, 22/32 for clarithromycin and 1/14 for telithromycin maintained high MIC values. In single-step studies, the frequency of mutations was -10 for H. influenzae and M. catarrhalis for telithromycin, azithromycin and clarithromycin. Telithromycin induced mutations at a lower rate than azithromycin and clarithromycin in streptococcal strains. Conclusion: Telithromycin showed a very limited ability to select for resistance in respiratory pathogens compared with azithromycin and clarithromycin.

KW - Azithromycin

KW - Clarithromycin

KW - In vitro selection of resistance

KW - Ketolides

KW - Respiratory pathogens

UR - http://www.scopus.com/inward/record.url?scp=4444241452&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4444241452&partnerID=8YFLogxK

U2 - 10.1093/jac/dkh339

DO - 10.1093/jac/dkh339

M3 - Article

C2 - 15215227

AN - SCOPUS:4444241452

VL - 54

SP - 542

EP - 545

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

IS - 2

ER -