Selective activation of cervical microvascular endothelial cells by human papillomavirus 16-E7 oncoprotein

Rossella D'Anna, Hélène Le Buanec, Giulio Alessandri, Arnaldo Caruso, Arsène Burny, Robert Gallo, Jean François Zagury, Daniel Zagury, Patrizia D'Alessio

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Human papillomavirus type 16 (HPV16) is strongly implicated in the etiology of cervical cancer, with the expression of HPV16-encoded E7 oncoprotein in infected epithelial cells contributing to their malignant transformation. Although nuclear E7 interacts with several nuclear targets, we have previously shown that extracellular E7 can cause suppression of immune cell function. Moreover, cervical microvascular endothelial (CrMVEn) cells treated with E7 increase their expression of adhesion molecules. High levels of some cytokines in serum and in cervicovaginal secretions are associated with the progression of cervical cancer. In this study, we investigated the effects of extracellular E7 on cytokine production and on cytoskeleton structure of CrMVEn cells and vascular endothelial cells from different organs. Methods: Immunocytochemical staining and flow cytometry techniques were used to detect E7 in endothelial cells incubated with purified E7 protein. Laser scanning confocal microscopy was used to study the E7-induced modification of the endothelial cytoskeleton. An enzyme-linked immunosorbent assay was performed to measure the production of two cytokines, interleukin 6 (IL-6) and interleukin 8 (IL-8), by E7-treated endothelial cells. All statistical tests were two-sided. Results: Extracellular E7 was taken up by CrMVEn cells and localized to the cytoplasm. CrMVEn cells showed a statistically significant (P

Original languageEnglish
Pages (from-to)1843-1851
Number of pages9
JournalJournal of the National Cancer Institute
Volume93
Issue number24
Publication statusPublished - Dec 19 2001

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Human papillomavirus 16
Oncogene Proteins
Endothelial Cells
Cytokines
Cytoskeleton
Uterine Cervical Neoplasms
Interleukin-8
Confocal Microscopy
Interleukin-6
Flow Cytometry
Cytoplasm
Epithelial Cells
Enzyme-Linked Immunosorbent Assay
Staining and Labeling
Serum

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

D'Anna, R., Le Buanec, H., Alessandri, G., Caruso, A., Burny, A., Gallo, R., ... D'Alessio, P. (2001). Selective activation of cervical microvascular endothelial cells by human papillomavirus 16-E7 oncoprotein. Journal of the National Cancer Institute, 93(24), 1843-1851.

Selective activation of cervical microvascular endothelial cells by human papillomavirus 16-E7 oncoprotein. / D'Anna, Rossella; Le Buanec, Hélène; Alessandri, Giulio; Caruso, Arnaldo; Burny, Arsène; Gallo, Robert; Zagury, Jean François; Zagury, Daniel; D'Alessio, Patrizia.

In: Journal of the National Cancer Institute, Vol. 93, No. 24, 19.12.2001, p. 1843-1851.

Research output: Contribution to journalArticle

D'Anna, R, Le Buanec, H, Alessandri, G, Caruso, A, Burny, A, Gallo, R, Zagury, JF, Zagury, D & D'Alessio, P 2001, 'Selective activation of cervical microvascular endothelial cells by human papillomavirus 16-E7 oncoprotein', Journal of the National Cancer Institute, vol. 93, no. 24, pp. 1843-1851.
D'Anna R, Le Buanec H, Alessandri G, Caruso A, Burny A, Gallo R et al. Selective activation of cervical microvascular endothelial cells by human papillomavirus 16-E7 oncoprotein. Journal of the National Cancer Institute. 2001 Dec 19;93(24):1843-1851.
D'Anna, Rossella ; Le Buanec, Hélène ; Alessandri, Giulio ; Caruso, Arnaldo ; Burny, Arsène ; Gallo, Robert ; Zagury, Jean François ; Zagury, Daniel ; D'Alessio, Patrizia. / Selective activation of cervical microvascular endothelial cells by human papillomavirus 16-E7 oncoprotein. In: Journal of the National Cancer Institute. 2001 ; Vol. 93, No. 24. pp. 1843-1851.
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