Selective Binding of shc-SH2 Domain to Tyrosine-Phosphorylated ζ- but Not γ-Chain upon CD16 Ligation on Human NK Cells

Ricciarda Galandrini, Gabriella Palmieri, Rossella Paolini, Mario Piccoli, Luigi Frati, Angela Santoni

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

FcγRIII (CD16) is a hetero-oligomeric receptor composed of a ligand-binding a subunit associated with homo- or heterodimers of the TCR ζ- and Fc∈RI γ-chains. We have previously demonstrated that CD16 ligation promotes complex formation between tyrosine-phosphorylated shc and Grb2, leading to activation of ras signaling pathway in human NK cells. Here we report that CD16 engagement induces rapid shc association with the tyrosine-phosphorylated receptor complex in human NK cells. In vitro binding studies demonstrate that this interaction is mediated by the shc-SH2 domain, and immunodepletion experiments indicate that the ζ- but not the γ-chain has the capability to mediate this association. Jurkat cell clones expressing CD16-ζ or -γ homodimers have been used to gain more information about the mechanism of shc/CD16 association. Our data show that, while engagement of both receptors induces tyrosine phosphorylation of shc and Grb2 recruitment, shc-SH2/receptor complex association is evident only in CD16-ζ but not in CD16-γ transfectants. Overall, our data demonstrate that the adaptor protein shc can be recruited to the activated CD16 complex by interaction with tyrosine-phosphorylated ζ-chain in a SH2-dependent manner. These results also provide further support to the notion that ζ- and γ-chains might couple to different biochemical pathways.

Original languageEnglish
Pages (from-to)3767-3773
Number of pages7
JournalJournal of Immunology
Volume159
Issue number8
Publication statusPublished - Oct 15 1997

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src Homology Domains
Natural Killer Cells
Ligation
Tyrosine
Shc Signaling Adaptor Proteins
Jurkat Cells
Clone Cells
Phosphorylation
Ligands
tyrosine receptor
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

Selective Binding of shc-SH2 Domain to Tyrosine-Phosphorylated ζ- but Not γ-Chain upon CD16 Ligation on Human NK Cells. / Galandrini, Ricciarda; Palmieri, Gabriella; Paolini, Rossella; Piccoli, Mario; Frati, Luigi; Santoni, Angela.

In: Journal of Immunology, Vol. 159, No. 8, 15.10.1997, p. 3767-3773.

Research output: Contribution to journalArticle

Galandrini, R, Palmieri, G, Paolini, R, Piccoli, M, Frati, L & Santoni, A 1997, 'Selective Binding of shc-SH2 Domain to Tyrosine-Phosphorylated ζ- but Not γ-Chain upon CD16 Ligation on Human NK Cells', Journal of Immunology, vol. 159, no. 8, pp. 3767-3773.
Galandrini, Ricciarda ; Palmieri, Gabriella ; Paolini, Rossella ; Piccoli, Mario ; Frati, Luigi ; Santoni, Angela. / Selective Binding of shc-SH2 Domain to Tyrosine-Phosphorylated ζ- but Not γ-Chain upon CD16 Ligation on Human NK Cells. In: Journal of Immunology. 1997 ; Vol. 159, No. 8. pp. 3767-3773.
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