Selective biochemical manipulation of twin neuronal networks on microelectrode arrays

Giulia Regalia, Emilia Biffi, Marco Rasponi, Alessandra Pedrocchi

Research output: Contribution to journalArticle

Abstract

Comparability of responses to neuroactive compounds and spatially and temporally resolved delivery of soluble factors are two major key features for pharmacological assays. Here, we describe the fabrication and the use of a device for long-term growth of twin neuronal networks and for their controlled biochemicalstimulation. The device is formed by a PDMS microfl uidic chamber coupled to a fl at Microelectrode Array (MEA), which provides the electrophysiological readout of the pharmacological stimulation. A partial physical barrier divides the chamber in two sub-compartments, where two functionally independent but fl uidically connected neuronal networks can be grown. This platform improves biological comparability between cultures and allows to perform selective and temporally controlled stimulations to neurons, running parallel pharmacological tests on the same device.

Original languageEnglish
Pages (from-to)217-230
Number of pages14
JournalNeuromethods
Volume103
DOIs
Publication statusPublished - 2015

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Microelectrodes
Neurons
Assays
Pharmacology
Fabrication
Equipment and Supplies
Architectural Accessibility
Growth

Keywords

  • Controlled biochemical stimulation
  • Drug delivery
  • Microelectrode arrays
  • Microfluidics
  • Microlithography
  • Neuronal culture
  • Neuropharmacology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Neuroscience(all)
  • Psychiatry and Mental health

Cite this

Selective biochemical manipulation of twin neuronal networks on microelectrode arrays. / Regalia, Giulia; Biffi, Emilia; Rasponi, Marco; Pedrocchi, Alessandra.

In: Neuromethods, Vol. 103, 2015, p. 217-230.

Research output: Contribution to journalArticle

Regalia, Giulia ; Biffi, Emilia ; Rasponi, Marco ; Pedrocchi, Alessandra. / Selective biochemical manipulation of twin neuronal networks on microelectrode arrays. In: Neuromethods. 2015 ; Vol. 103. pp. 217-230.
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