Selective enrichment in human megakaryocyte progenitors by the B203.13 surface differentiation antigen

Marco Vitale; Giorgio Zauli; Alessandra Bassini; Giuseppe Visani; Rita Rezzani; Carlo M. Croce; Francesco Antonio Manzoli

Selective enrichment in human megakaryocyte progenitors by the B203.13 surface differentiation antigen

Marco Vitale, Giorgio Zauli, Alessandra Bassini, Giuseppe Visani, Rita Rezzani, Carlo M. Croce, Francesco Antonio Manzoli

IRCCS pediatrico Burlo Garofolo

Research output: Contribution to journal › Article › peer-review

Abstract

The B203.13 monoclonal antibody specifically recognizes a subset (8- 18.5%) of human CD34⁺ haemopoietic progenitors, which are significantly (P+/B203.13⁺ showed a greater number of cells/colony with respect to CD34⁺/B203.13^-; (ii) after 10d of liquid cultures with 100ng/ml of thrombopoietin, only a minority of CD34-derived cells positive for CD41 coexpressed B203.13; (iii) in situ immunocytochemical staining revealed that B203.13 was expressed exclusively on immature megakaryoblasts; (iv) circulating platelets did not express B203.13.

Original language	English
Pages (from-to)	766-769
Number of pages	4
Journal	British Journal of Haematology
Volume	99
Issue number	4
Publication status	Published - 1997

Keywords

Cell differentiation
Megakaryocytes
Platelets

ASJC Scopus subject areas

Hematology

Cite this

Selective enrichment in human megakaryocyte progenitors by the B203.13 surface differentiation antigen. / Vitale, Marco; Zauli, Giorgio; Bassini, Alessandra; Visani, Giuseppe; Rezzani, Rita; Croce, Carlo M.; Manzoli, Francesco Antonio.

In: British Journal of Haematology, Vol. 99, No. 4, 1997, p. 766-769.

Research output: Contribution to journal › Article › peer-review

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abstract = "The B203.13 monoclonal antibody specifically recognizes a subset (8- 18.5%) of human CD34+ haemopoietic progenitors, which are significantly (P+/B203.13+ showed a greater number of cells/colony with respect to CD34+/B203.13-; (ii) after 10d of liquid cultures with 100ng/ml of thrombopoietin, only a minority of CD34-derived cells positive for CD41 coexpressed B203.13; (iii) in situ immunocytochemical staining revealed that B203.13 was expressed exclusively on immature megakaryoblasts; (iv) circulating platelets did not express B203.13.",

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AU - Vitale, Marco

AU - Zauli, Giorgio

AU - Bassini, Alessandra

AU - Visani, Giuseppe

AU - Rezzani, Rita

AU - Croce, Carlo M.

AU - Manzoli, Francesco Antonio

PY - 1997

Y1 - 1997

N2 - The B203.13 monoclonal antibody specifically recognizes a subset (8- 18.5%) of human CD34+ haemopoietic progenitors, which are significantly (P+/B203.13+ showed a greater number of cells/colony with respect to CD34+/B203.13-; (ii) after 10d of liquid cultures with 100ng/ml of thrombopoietin, only a minority of CD34-derived cells positive for CD41 coexpressed B203.13; (iii) in situ immunocytochemical staining revealed that B203.13 was expressed exclusively on immature megakaryoblasts; (iv) circulating platelets did not express B203.13.

AB - The B203.13 monoclonal antibody specifically recognizes a subset (8- 18.5%) of human CD34+ haemopoietic progenitors, which are significantly (P+/B203.13+ showed a greater number of cells/colony with respect to CD34+/B203.13-; (ii) after 10d of liquid cultures with 100ng/ml of thrombopoietin, only a minority of CD34-derived cells positive for CD41 coexpressed B203.13; (iii) in situ immunocytochemical staining revealed that B203.13 was expressed exclusively on immature megakaryoblasts; (iv) circulating platelets did not express B203.13.

KW - Cell differentiation

KW - Megakaryocytes

KW - Platelets

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Selective enrichment in human megakaryocyte progenitors by the B203.13 surface differentiation antigen

Abstract

Keywords

ASJC Scopus subject areas

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