Selective expression of high molecular weight basic fibroblast growth factor confers a unique phenotype to NIH 3T3 cells

Natalina Quarto, Daniela Talarico, Robert Florkiewicz, Daniel B. Rifkin

Research output: Contribution to journalArticle

Abstract

The phenotypes of NIH 3T3 cells transfected with basic fibroblast growth factor (bFGF) cDNAs that express only the high molecular weight (HMW) forms of bFGF, the 18-kDa form, or all forms were examined. Cells producing the 18 kDa or all forms of bFGF were transformed at high levels of growth factor expression but were nontransformed at low levels. Cell producing low levels of HMW forms of bFGF were growth impaired when compared with the parental cells. These cells tended to form multinucleated giant cells, did not grow in soft agar, were nontumorigenic, had a normal bFGF receptor number, and had a nontransformed morphology. Cells expressing high levels of HMW bFGFs had a transformed morphology and were tumorigenic. These data suggest a specific functional role for HMWbFGF.

Original languageEnglish
Pages (from-to)699-708
Number of pages10
JournalMolecular Biology of the Cell
Volume2
Issue number9
Publication statusPublished - Sep 1991

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

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