Selective Infection of Antigen-Specific B Lymphocytes by Salmonella Mediates Bacterial Survival and Systemic Spreading of Infection

Yuri Souwer, Alexander Griekspoor, Jelle de Wit, Chiara Martinoli, Elena Zagato, Hans Janssen, Tineke Jorritsma, Yotam E. Bar-Ephraïm, Maria Rescigno, Jacques Neefjes, S. Marieke van Ham

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Abstract

Background: The bacterial pathogen Salmonella causes worldwide disease. A major route of intestinal entry involves M cells, providing access to B cell-rich Peyer's Patches. Primary human B cells phagocytose Salmonella typhimurium upon recognition by the specific surface Ig receptor (BCR). As it is unclear how Salmonella disseminates systemically, we studied whether Salmonella can use B cells as a transport device for spreading. Methodology/Principal Findings: Human primary B cells or Ramos cell line were incubated with GFP-expressing Salmonella. Intracellular survival and escape was studied in vitro by live cell imaging, flow cytometry and flow imaging. HEL-specific B cells were transferred into C57BL/6 mice and HEL-expressing Salmonella spreading in vivo was analyzed investigating mesenteric lymph nodes, spleen and blood. After phagocytosis by B cells, Salmonella survives intracellularly in a non-replicative state which is actively maintained by the B cell. Salmonella is later excreted followed by reproductive infection of other cell types. Salmonella-specific B cells thus act both as a survival niche and a reservoir for reinfection. Adoptive transfer of antigen-specific B cells before oral infection of mice showed that these B cells mediate in vivo systemic spreading of Salmonella to spleen and blood. Conclusions/Significance: This is a first example of a pathogenic bacterium that abuses the antigen-specific cells of the adaptive immune system for systemic spreading for dissemination of infection.

Original languageEnglish
Article numbere50667
JournalPLoS One
Volume7
Issue number11
DOIs
Publication statusPublished - Nov 29 2012

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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    Souwer, Y., Griekspoor, A., de Wit, J., Martinoli, C., Zagato, E., Janssen, H., Jorritsma, T., Bar-Ephraïm, Y. E., Rescigno, M., Neefjes, J., & van Ham, S. M. (2012). Selective Infection of Antigen-Specific B Lymphocytes by Salmonella Mediates Bacterial Survival and Systemic Spreading of Infection. PLoS One, 7(11), [e50667]. https://doi.org/10.1371/journal.pone.0050667