Selective inhibition of carbonic anhydrase IX decreases cell proliferation and induces ceramide-mediated apoptosis in human cancer cells

Fabio Cianchi, Maria Cristina Vinci, Claudiu T. Supuran, Benedetta Peruzzi, Paolo De Giuli, Giuseppe Fasolis, Giuliano Perigli, Silvia Pastorekova, Laura Papucci, Alessandro Pini, Emanuela Masini, Luca Puccetti

Research output: Contribution to journalArticle

Abstract

Recently, carbonic anhydrase (CA) inhibitors have been proposed as a potential new class of antitumor agents. The aim of this study was to evaluate the antitumor activity of three CA inhibitors, namely acetazolamide (AZ) and two newly synthesized aromatic sulfonamides with high affinity forCAIX, 2-(4-sulfamoylphenyl-amino)-4,6-dichloro-1,3,5-triazine (TR1) and 4-[3-(N,N-dimethylaminopropyl)thioreido-phenylsulfonylaminoethyl] benzenesulfonamide (GA15), against human tumor cells. The effects of AZ, TR1, and GA15 on cell proliferation and apoptosis were evaluated in CA IX-positive HeLa and 786-O cells andCAIX-negative 786-O/von Hippel-Lindau (VHL) cells. We also investigated whether the potential antitumor activity of these molecules might be mediated by an increase in ceramide production. AZ, TR1, and GA15 could significantly reduce cell proliferation and induce apoptosis in HeLa and 786-O cells. Moreover, all three inhibitors could decrease intracellular pH (pH i) and increase ceramide production in the same cells. Treatment with the ceramide synthase inhibitor fumonisin B1 prevented the apoptotic effects of the three CA inhibitors. In all experiments, the effects of aromatic sulfonamides were more pronounced than those of AZ. The three inhibitors did not show any antitumor activity in CA IX-negative 786-O/VHL cells and failed to lower pHi or increase intracellular ceramide levels in the same cells. In conclusion, CA inhibition can decrease cell proliferation and induce apoptosis in human tumor cells. The ability of CA inhibitors to decrease pH i might trigger cell apoptosis through mediation of ceramide synthesis. Activation of this apoptotic cascade probably is mediated by inhibition of the CA IX isoform.

Original languageEnglish
Pages (from-to)710-719
Number of pages10
JournalJournal of Pharmacology and Experimental Therapeutics
Volume334
Issue number3
DOIs
Publication statusPublished - 2010

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Ceramides
Cell Proliferation
Apoptosis
Carbonic Anhydrase Inhibitors
Acetazolamide
Neoplasms
Sulfonamides
Carbonic Anhydrase IX
Triazines
Carbonic Anhydrases
Antineoplastic Agents
Protein Isoforms

ASJC Scopus subject areas

  • Pharmacology
  • Molecular Medicine
  • Medicine(all)

Cite this

Selective inhibition of carbonic anhydrase IX decreases cell proliferation and induces ceramide-mediated apoptosis in human cancer cells. / Cianchi, Fabio; Vinci, Maria Cristina; Supuran, Claudiu T.; Peruzzi, Benedetta; De Giuli, Paolo; Fasolis, Giuseppe; Perigli, Giuliano; Pastorekova, Silvia; Papucci, Laura; Pini, Alessandro; Masini, Emanuela; Puccetti, Luca.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 334, No. 3, 2010, p. 710-719.

Research output: Contribution to journalArticle

Cianchi, F, Vinci, MC, Supuran, CT, Peruzzi, B, De Giuli, P, Fasolis, G, Perigli, G, Pastorekova, S, Papucci, L, Pini, A, Masini, E & Puccetti, L 2010, 'Selective inhibition of carbonic anhydrase IX decreases cell proliferation and induces ceramide-mediated apoptosis in human cancer cells', Journal of Pharmacology and Experimental Therapeutics, vol. 334, no. 3, pp. 710-719. https://doi.org/10.1124/jpet.110.167270
Cianchi, Fabio ; Vinci, Maria Cristina ; Supuran, Claudiu T. ; Peruzzi, Benedetta ; De Giuli, Paolo ; Fasolis, Giuseppe ; Perigli, Giuliano ; Pastorekova, Silvia ; Papucci, Laura ; Pini, Alessandro ; Masini, Emanuela ; Puccetti, Luca. / Selective inhibition of carbonic anhydrase IX decreases cell proliferation and induces ceramide-mediated apoptosis in human cancer cells. In: Journal of Pharmacology and Experimental Therapeutics. 2010 ; Vol. 334, No. 3. pp. 710-719.
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AU - Cianchi, Fabio

AU - Vinci, Maria Cristina

AU - Supuran, Claudiu T.

AU - Peruzzi, Benedetta

AU - De Giuli, Paolo

AU - Fasolis, Giuseppe

AU - Perigli, Giuliano

AU - Pastorekova, Silvia

AU - Papucci, Laura

AU - Pini, Alessandro

AU - Masini, Emanuela

AU - Puccetti, Luca

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AB - Recently, carbonic anhydrase (CA) inhibitors have been proposed as a potential new class of antitumor agents. The aim of this study was to evaluate the antitumor activity of three CA inhibitors, namely acetazolamide (AZ) and two newly synthesized aromatic sulfonamides with high affinity forCAIX, 2-(4-sulfamoylphenyl-amino)-4,6-dichloro-1,3,5-triazine (TR1) and 4-[3-(N,N-dimethylaminopropyl)thioreido-phenylsulfonylaminoethyl] benzenesulfonamide (GA15), against human tumor cells. The effects of AZ, TR1, and GA15 on cell proliferation and apoptosis were evaluated in CA IX-positive HeLa and 786-O cells andCAIX-negative 786-O/von Hippel-Lindau (VHL) cells. We also investigated whether the potential antitumor activity of these molecules might be mediated by an increase in ceramide production. AZ, TR1, and GA15 could significantly reduce cell proliferation and induce apoptosis in HeLa and 786-O cells. Moreover, all three inhibitors could decrease intracellular pH (pH i) and increase ceramide production in the same cells. Treatment with the ceramide synthase inhibitor fumonisin B1 prevented the apoptotic effects of the three CA inhibitors. In all experiments, the effects of aromatic sulfonamides were more pronounced than those of AZ. The three inhibitors did not show any antitumor activity in CA IX-negative 786-O/VHL cells and failed to lower pHi or increase intracellular ceramide levels in the same cells. In conclusion, CA inhibition can decrease cell proliferation and induce apoptosis in human tumor cells. The ability of CA inhibitors to decrease pH i might trigger cell apoptosis through mediation of ceramide synthesis. Activation of this apoptotic cascade probably is mediated by inhibition of the CA IX isoform.

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