Selective inhibition of HIV replication in primary macrophages but not T lymphocytes by macrophage-derived chemokine

Manuela Cota, Manuela Mengozzi, Elisa Vicenzi, Paola Panina-Bordignon, Francesco Sinigaglia, Pietro Transidico, Silvano Sozzani, Alberto Mantovani, Guido Poli

Research output: Contribution to journalArticle

Abstract

Macrophage-derived chemokine (MDC) has been reported to inhibit different HIV-1 strains in activated peripheral blood mononuclear cells (T cell blasts), although other investigators have not confirmed these findings. Here we demonstrate that MDC inhibits the replication of CCR5-dependent (R5) HIV-1(BaL) in monocytederived macrophages (MDM), but not in T cell blasts, although with variable potency depending on donor variability. Analysis of HIV-1(BaL) proviral DNA synthesis in MDM indicated that the suppressive effect of MDC did not involve inhibition of early events such as entry or reverse transcription. Finally, an inverse correlation was observed between the levels of endogenous MDC secreted by uninfected MDM of different donors and the efficiency of different HIV strains, including two primary isolates with different coreceptor usage, to replicate in these cells. Thus, MDC represents an example of a chemokine inhibiting HIV replication in macrophages acting at one or more postentry levels in the virus life cycle.

Original languageEnglish
Pages (from-to)9162-9167
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number16
DOIs
Publication statusPublished - Aug 1 2000

ASJC Scopus subject areas

  • Genetics
  • General

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