Selective inhibition of IgE versus β2-microglobulin in human U-266 myeloma cell line treated with T-cell-derived factors

P. Rossi, E. Galli, M. Gidlund, Z. Salahuddin, K. Laan, H. Wigzell

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Concanavalin-A-activated T cells and their crude supernatants were assayed for suppressive activity on an IgE-producing U-266 cell line. Detectable and comparable degrees of suppression were obtained with the co-culture and the supernatant protocols. Separation of the effector population into T4+ and T8+ subsets showed the most effective cells in the T8+ fraction. Control experiments demonstrated that the IgE down-regulation was selective, since parallel measurement of β2-microglobulin synthesis showed no effect of T cells or T-cell-derived supernatants. In addition, several human T-cell lymphoma-leukaemia virus I-transformed T-cell lines were explored for their capacity to produce factor(s) able to suppress IgE synthesis in the U-266 cell line, and four out of 25 cell lines could be shown to do this in a constitutive manner. Kinetic studies suggested that the inhibition occurred at a transcriptional level. The results indicate that the T-cell-myeloma system is an interesting model to define better the regulation of IgE in the human.

Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalScandinavian Journal of Immunology
Volume22
Issue number1
DOIs
Publication statusPublished - 1985

Fingerprint

TCF Transcription Factors
Immunoglobulin E
T-Lymphocytes
Cell Line
CD8-Positive T-Lymphocytes
Transformed Cell Line
Human T-lymphotropic virus 1
T-Cell Lymphoma
Concanavalin A
Coculture Techniques
Down-Regulation
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Selective inhibition of IgE versus β2-microglobulin in human U-266 myeloma cell line treated with T-cell-derived factors. / Rossi, P.; Galli, E.; Gidlund, M.; Salahuddin, Z.; Laan, K.; Wigzell, H.

In: Scandinavian Journal of Immunology, Vol. 22, No. 1, 1985, p. 33-39.

Research output: Contribution to journalArticle

Rossi, P. ; Galli, E. ; Gidlund, M. ; Salahuddin, Z. ; Laan, K. ; Wigzell, H. / Selective inhibition of IgE versus β2-microglobulin in human U-266 myeloma cell line treated with T-cell-derived factors. In: Scandinavian Journal of Immunology. 1985 ; Vol. 22, No. 1. pp. 33-39.
@article{0172e0e18d7949ad91942fb1b0a3c077,
title = "Selective inhibition of IgE versus β2-microglobulin in human U-266 myeloma cell line treated with T-cell-derived factors",
abstract = "Concanavalin-A-activated T cells and their crude supernatants were assayed for suppressive activity on an IgE-producing U-266 cell line. Detectable and comparable degrees of suppression were obtained with the co-culture and the supernatant protocols. Separation of the effector population into T4+ and T8+ subsets showed the most effective cells in the T8+ fraction. Control experiments demonstrated that the IgE down-regulation was selective, since parallel measurement of β2-microglobulin synthesis showed no effect of T cells or T-cell-derived supernatants. In addition, several human T-cell lymphoma-leukaemia virus I-transformed T-cell lines were explored for their capacity to produce factor(s) able to suppress IgE synthesis in the U-266 cell line, and four out of 25 cell lines could be shown to do this in a constitutive manner. Kinetic studies suggested that the inhibition occurred at a transcriptional level. The results indicate that the T-cell-myeloma system is an interesting model to define better the regulation of IgE in the human.",
author = "P. Rossi and E. Galli and M. Gidlund and Z. Salahuddin and K. Laan and H. Wigzell",
year = "1985",
doi = "10.1111/j.1365-3083.1985.tb01857.x",
language = "English",
volume = "22",
pages = "33--39",
journal = "Scandinavian Journal of Immunology",
issn = "0300-9475",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Selective inhibition of IgE versus β2-microglobulin in human U-266 myeloma cell line treated with T-cell-derived factors

AU - Rossi, P.

AU - Galli, E.

AU - Gidlund, M.

AU - Salahuddin, Z.

AU - Laan, K.

AU - Wigzell, H.

PY - 1985

Y1 - 1985

N2 - Concanavalin-A-activated T cells and their crude supernatants were assayed for suppressive activity on an IgE-producing U-266 cell line. Detectable and comparable degrees of suppression were obtained with the co-culture and the supernatant protocols. Separation of the effector population into T4+ and T8+ subsets showed the most effective cells in the T8+ fraction. Control experiments demonstrated that the IgE down-regulation was selective, since parallel measurement of β2-microglobulin synthesis showed no effect of T cells or T-cell-derived supernatants. In addition, several human T-cell lymphoma-leukaemia virus I-transformed T-cell lines were explored for their capacity to produce factor(s) able to suppress IgE synthesis in the U-266 cell line, and four out of 25 cell lines could be shown to do this in a constitutive manner. Kinetic studies suggested that the inhibition occurred at a transcriptional level. The results indicate that the T-cell-myeloma system is an interesting model to define better the regulation of IgE in the human.

AB - Concanavalin-A-activated T cells and their crude supernatants were assayed for suppressive activity on an IgE-producing U-266 cell line. Detectable and comparable degrees of suppression were obtained with the co-culture and the supernatant protocols. Separation of the effector population into T4+ and T8+ subsets showed the most effective cells in the T8+ fraction. Control experiments demonstrated that the IgE down-regulation was selective, since parallel measurement of β2-microglobulin synthesis showed no effect of T cells or T-cell-derived supernatants. In addition, several human T-cell lymphoma-leukaemia virus I-transformed T-cell lines were explored for their capacity to produce factor(s) able to suppress IgE synthesis in the U-266 cell line, and four out of 25 cell lines could be shown to do this in a constitutive manner. Kinetic studies suggested that the inhibition occurred at a transcriptional level. The results indicate that the T-cell-myeloma system is an interesting model to define better the regulation of IgE in the human.

UR - http://www.scopus.com/inward/record.url?scp=0021831065&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021831065&partnerID=8YFLogxK

U2 - 10.1111/j.1365-3083.1985.tb01857.x

DO - 10.1111/j.1365-3083.1985.tb01857.x

M3 - Article

VL - 22

SP - 33

EP - 39

JO - Scandinavian Journal of Immunology

JF - Scandinavian Journal of Immunology

SN - 0300-9475

IS - 1

ER -