Selective inhibition of miR-92 in hippocampal neurons alters contextual fear memory

Gisella Vetere, Christian Barbato, Silvia Pezzola, Paola Frisone, Massimiliano Aceti, Mariateresa Ciotti, Carlo Cogoni, Martine Ammassari-Teule, Francesca Ruberti

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Post-transcriptional gene regulation mediated by microRNAs (miRNAs) is implicated in memory formation; however, the function of miR-92 in this regulation is uncharacterized. The present study shows that training mice in contextual fear conditioning produces a transient increase in miR-92 levels in the hippocampus and decreases several miR-92 gene targets, including: (i) the neuronal Cl- extruding K+Cl- co-transporter 2 (KCC2) protein; (ii) the cytoplasmic polyadenylation protein (CPEB3), an RNA-binding protein regulator of protein synthesis in neurons; and (iii) the transcription factor myocyte enhancer factor 2D (MEF2D), one of the MEF2 genes which negatively regulates memory-induced structural plasticity. Selective inhibition of endogenous miR-92 in CA1 hippocampal neurons, by a sponge lentiviral vector expressing multiple sequences imperfectly complementary to mature miR-92 under the control of the neuronal specific synapsin promoter, leads to up-regulation of KCC2, CPEB3 and MEF2D, impairs contextual fear conditioning, and prevents a memory-induced increase in the spine density. Taken together, the results indicate that neuronal-expressed miR-92 is an endogenous fine regulator of contextual fear memory in mice.

Original languageEnglish
Pages (from-to)1458-1465
Number of pages8
JournalHippocampus
Volume24
Issue number12
DOIs
Publication statusPublished - Dec 1 2014

Fingerprint

Fear
MEF2 Transcription Factors
Symporters
Neurons
Synapsins
Genes
Polyadenylation
Proteins
RNA-Binding Proteins
Porifera
MicroRNAs
Hippocampus
Spine
Transcription Factors
Up-Regulation
Inhibition (Psychology)
Conditioning (Psychology)

Keywords

  • Contextual fear conditioning
  • Dendritic spines
  • Hippocampus
  • MicroRNA
  • MiR-92

ASJC Scopus subject areas

  • Cognitive Neuroscience
  • Medicine(all)

Cite this

Vetere, G., Barbato, C., Pezzola, S., Frisone, P., Aceti, M., Ciotti, M., ... Ruberti, F. (2014). Selective inhibition of miR-92 in hippocampal neurons alters contextual fear memory. Hippocampus, 24(12), 1458-1465. https://doi.org/10.1002/hipo.22326

Selective inhibition of miR-92 in hippocampal neurons alters contextual fear memory. / Vetere, Gisella; Barbato, Christian; Pezzola, Silvia; Frisone, Paola; Aceti, Massimiliano; Ciotti, Mariateresa; Cogoni, Carlo; Ammassari-Teule, Martine; Ruberti, Francesca.

In: Hippocampus, Vol. 24, No. 12, 01.12.2014, p. 1458-1465.

Research output: Contribution to journalArticle

Vetere, G, Barbato, C, Pezzola, S, Frisone, P, Aceti, M, Ciotti, M, Cogoni, C, Ammassari-Teule, M & Ruberti, F 2014, 'Selective inhibition of miR-92 in hippocampal neurons alters contextual fear memory', Hippocampus, vol. 24, no. 12, pp. 1458-1465. https://doi.org/10.1002/hipo.22326
Vetere G, Barbato C, Pezzola S, Frisone P, Aceti M, Ciotti M et al. Selective inhibition of miR-92 in hippocampal neurons alters contextual fear memory. Hippocampus. 2014 Dec 1;24(12):1458-1465. https://doi.org/10.1002/hipo.22326
Vetere, Gisella ; Barbato, Christian ; Pezzola, Silvia ; Frisone, Paola ; Aceti, Massimiliano ; Ciotti, Mariateresa ; Cogoni, Carlo ; Ammassari-Teule, Martine ; Ruberti, Francesca. / Selective inhibition of miR-92 in hippocampal neurons alters contextual fear memory. In: Hippocampus. 2014 ; Vol. 24, No. 12. pp. 1458-1465.
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